Accéder au contenu
Merck
  • Metabolites from cerebrospinal fluid in aneurysmal subarachnoid haemorrhage correlate with vasospasm and clinical outcome: a pattern-recognition 1H NMR study.

Metabolites from cerebrospinal fluid in aneurysmal subarachnoid haemorrhage correlate with vasospasm and clinical outcome: a pattern-recognition 1H NMR study.

NMR in biomedicine (2004-09-30)
Victoria G Dunne, Shermina Bhattachayya, Michael Besser, Caroline Rae, Julian L Griffin
RÉSUMÉ

Following subarachnoid haemorrhage the most significant complication is sustained cerebral vascular contraction (vasospasm), which may result in terminal brain damage from cerebral infarction. Despite this, the biochemical cause of vasospasm remains poorly understood. In this study, the global high-concentration metabolite composition of CSF has been correlated with patient outcome after subarachnoid haemorrhage using multivariate statistics and 1H NMR spectroscopy. In total, 16 patients with aneurysmal subarachnoid haemorrhage (aSAH) were compared with 16 control patients who required a procedure where CSF was obtained but did not have aSAH. Multivariate statistics readily distinguished the aSAH group from the heterogeneous control group, even when only those controls with blood contamination in the CSF were used. Using principal components analysis and orthogonal signal correction, vasospasm was correlated to the concentrations of lactate, glucose and glutamine. These pattern recognition models of the NMR data also predicted Glasgow Coma Score (54% within +/- 1 of the actual score on a scale of 1-15 for the whole patient group), Hunt and Hess SAH severity score (88% within +/- 1 of the actual score on a scale of 1-5 for the aSAH group) and cognitive outcome scores (78% within +/- 3 of the actual score on a 100% scale for the whole patient group). Thus, the approach allowed the prediction of outcome as well as confirming the presence of aSAH.

MATÉRIAUX
Référence du produit
Marque
Description du produit

Sigma-Aldrich
Acide formique, reagent grade, ≥95%
Sigma-Aldrich
Acide formique, ACS reagent, ≥96%
Sigma-Aldrich
Acide formique, puriss. p.a., ACS reagent, reag. Ph. Eur., ≥98%
Sigma-Aldrich
Acide formique, puriss., meets analytical specifications of DAC, FCC, 98.0-100%
Sigma-Aldrich
Acide formique, ACS reagent, ≥88%
Sigma-Aldrich
myo-inositol, ≥99% (GC), BioReagent
Sigma-Aldrich
Adenosine, ≥99%
Sigma-Aldrich
myo-inositol, ≥99%
Sigma-Aldrich
Adenosine, suitable for cell culture, BioReagent
Sigma-Aldrich
Acide formique, ≥95%, FCC, FG
Sigma-Aldrich
Adenosine
Sigma-Aldrich
Acide formique solution, BioUltra, 1.0 M in H2O
Millipore
myo-inositol, suitable for microbiology, ≥99.0%
Supelco
Adenosine, Pharmaceutical Secondary Standard; Certified Reference Material
Adenosine, European Pharmacopoeia (EP) Reference Standard