Accéder au contenu
Merck

Reactivity of beta-carotene towards peroxyl radicals studied by laser flash and steady-state photolysis.

FEBS letters (1998-05-26)
A Mortensen, L H Skibsted
RÉSUMÉ

Peroxyl radicals, as model for peroxyl radicals formed during autoxidation of lipids, have been generated in three solvent systems (cyclohexane, tetrahydrofuran and tert-butanol/water) by steady-state and laser flash photolysis, and their reaction with beta-carotene studied. Steady-state photolysis experiments showed that alkyl, alkoxyl and alkylperoxyl radicals all react with beta-carotene. However, laser flash photolysis experiments indicated that the reaction with peroxyl radicals (second-order rate constant estimated to be less than 10(6) M(-1) s(-1)) is slower than with alkyl and alkoxyl radicals, and that beta-carotene is hence a poor direct scavenger of peroxyl radicals. Scavenging of peroxyl radicals by beta-carotene is suggested not to proceed via electron transfer but rather by adduct formation and/or hydrogen abstraction. For different phenoxyl radicals, differences in reactivity towards beta-carotene seem to be correlated with standard reduction potential.

MATÉRIAUX
Référence du produit
Marque
Description du produit

Sigma-Aldrich
Luperox® DI, tert-Butyl peroxide, 98%