- High-throughput screening for agonists of ROS production in live human vascular endothelial cells.
High-throughput screening for agonists of ROS production in live human vascular endothelial cells.
STAR protocols (2022-01-11)
Tomoya Sasahara, Minako Hoshi
PMID35005635
RÉSUMÉ
Reactive oxygen species (ROS) are important physiological molecules, and identifying agonists for ROS production can yield useful tools for future research. Here we present an optimized protocol for high-throughput screening for agonists that induce ROS production. We describe the use of a fluorescent probe in human vascular endothelial cells, which can establish whether ROS production occurs in mitochondria or in the plasma membrane of live cells. For complete details on the use and execution of this profile, please refer to Sasahara et al. (2021).
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Apocynin, A cell-permeable, anti-inflammatory phenolic compound that acts as a potent and selective inhibitor of NADPH oxidase.