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Y1251

Millipore

Yeast Nitrogen Base Without Amino Acids and Ammonium Sulfate

suitable for microbiology, NutriSelect® Basic

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About This Item

Code UNSPSC :
41171606
Nomenclature NACRES :
NA.85

Stérilité

non-sterile

Forme

powder

Conditionnement

poly bottle of 1 kg
poly bottle of 500 g

Fabricant/nom de marque

NutriSelect® Basic

Solubilité

water: soluble 67 mg/mL

Application(s)

clinical testing
food and beverages
life science and biopharma
microbiology

Adéquation

Candida spp.
Pichia spp.
Saccharomyces spp.
Zygosaccharomyces spp.
molds
yeasts

Description générale

Yeast Nitrogen Base Without Amino Acids and Ammonium Sulfate is recommended to use for classifying yeasts based on their carbon and nitrogen requirements. The medium contains all essential nutrients required for the growth of yeasts except amino acids, histidine, methionine and tryptophan, and also ammonium sulphate. The addition of nitrogen and carbon sources is required as described in the Manual of Clinical Microbiology

Application

Yeast Nitrogen Base Without Amino Acids and Ammonium Sulfate is used in classifying yeasts based on carbon and nitrogen requirements. It contains all essential nutrients required for the growth of yeasts except amino acids, nitrogen and carbohydrate. The addition of nitrogen and carbon sources is required.

Composants

Ingredients
Biotin, 2 μg/L
Calcium pantothenate, 400 μg/L
Folic acid, 2 μg/L
Niacin, 400 μg/L
p-Aminobenzoic acid, 200 μg/L
Pyridoxine HCl, 400 μg/L
Riboflavin, 200 μg/L
Thiamine HCl, 400 μg/L
Inositol, 2 mg/L
Boric acid, 500 μg/L
Copper sulfate, 40 μg/L
Potassium iodide, 100 μg/L
Ferric chloride, 200 μg/L
Manganese sulfate, 400 μg/L
Sodium molybdate, 200 μg/L
Zinc sulfate, 400 μg/L
Potassium phosphate monobasic, 1 g/L
Magnesium sulfate, 0.5 g/L
Sodium chloride, 0.1 g/L
Calcium chloride, 0.1 g/L

Notes préparatoires

  • Prepare a 10× stock solution by suspending 1.7 g of yeast nitrogen base without amino acids and ammonium sulfate in 100 ml of cold distilled water. Include desired nitrogen and carbon sources.
  • Warm if necessary to solubilize and sterilize by filtration.
  • Store 10× stock solution at 2-8°C. To use, dilute 1:10 with sterile distilled water under aspetic conditions.

Note de bas de page

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Informations légales

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Composants de kit seuls

Réf. du produit
Description
  • KH2PO4 1 g/L
  • MgSO4 .5 g/L
  • NaCl .1 g/L
  • CaCl2 .1 g/L
  • Inositol 2 mg/L

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, type N95 (US)

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Michael Mülleder et al.
Cell, 167(2), 553-565 (2016-10-04)
Genome-metabolism interactions enable cell growth. To probe the extent of these interactions and delineate their functional contributions, we quantified the Saccharomyces amino acid metabolome and its response to systematic gene deletion. Over one-third of coding genes, in particular those important for chromatin dynamics
Sviatlana Shashkova et al.
Bio-protocol, 8(2), e2710-e2710 (2018-02-13)
Single-molecule fluorescence microscopy enables unrivaled sub-cellular quantitation of genomically encoded fusions of native proteins with fluorescent protein reporters. Fluorescent proteins must undergo in vivo maturation after expression before they become photoactive. Maturation effects must be quantified during single-molecule analysis. Here
Yuping Lin et al.
Biotechnology for biofuels, 7(1), 126-126 (2014-12-02)
Saccharomyces cerevisiae, a key organism used for the manufacture of renewable fuels and chemicals, has been engineered to utilize non-native sugars derived from plant cell walls, such as cellobiose and xylose. However, the rates and efficiencies of these non-native sugar
Ligia Acosta-Sampson et al.
The Journal of biological chemistry, 292(48), 19610-19627 (2017-10-04)
Targeting of most integral membrane proteins to the endoplasmic reticulum is controlled by the signal recognition particle, which recognizes a hydrophobic signal sequence near the protein N terminus. Proper folding of these proteins is monitored by the unfolded protein response
Owen W Ryan et al.
eLife, 3 (2014-08-21)
The directed evolution of biomolecules to improve or change their activity is central to many engineering and synthetic biology efforts. However, selecting improved variants from gene libraries in living cells requires plasmid expression systems that suffer from variable copy number
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