SRP3327

IL-1 RECEPTOR ANTAGONIST human

Animal-component free, recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC)

• Synonyme(s) : ICIL-1RA, IL-1RN, IL-1Ra, IRAP
• Code UNSPSC : 12352200
• Nomenclature NACRES : NA.32

Propriétés

• Source biologique: human
• Produit recombinant: expressed in E. coli
• Pureté: ≥98% (HPLC); ≥98% (SDS-PAGE)
• Forme: lyophilized
• Puissance: 20-40 ng/mL
• Poids mol.: 17.2 kDa
• Conditionnement: pkg of 100 μg
• Conditions de stockage: (Keep container tightly closed in a dry and well-ventilated place)
• Technique(s): activity assay: suitable
• Impuretés: endotoxin, tested
• Couleur: white to off-white
• Numéro d'accès UniProt: P14778
• Conditions d'expédition: wet ice
• Température de stockage: −20°C
• Informations sur le gène: human ... IL1R1(3554)

Description

Description générale

Research area: Immunology & cytokines

Interleukin-1 receptor antagonist (IL-1RA) is a naturally occurring inflammatory inhibitor protein. IL-1Ra is produced by corneal epithelial cells, monocytes, neutrophils, macrophages, and fibroblasts. Recombinant human IL-1Ra is a 17.2kDa protein consisting of 153 amino acid residues.

Application

Interleukin-1 receptor antagonist (IL-1RA) human has been used:

• to study the role of interleukin (IL)-1β receptor in cell senescence induced by doxorubicin
• to block the IL-1β binding to its receptor
• to understand the role of autocrine interleukin-1β (IL-1β) signaling downstream of cl- in RPS27 modulation

Actions biochimiques/physiologiques

Interleukin-1 receptor antagonist (IL-1RA) inhibits the activity of IL-1α and IL-1β by competitively blocking binding to their type I and type II receptors. Therapeutically, IL-1RA may help in the treatment of sepsis, cachexia, rheumatoid arthritis, chronic myelogenous leukemia, asthma, psoriasis, and inflammatory bowel disease.

Forme physique

Lyophilized from 20 mM Tris, pH 8.0.

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable