SAB4200760

Anti-Fibronectin antibody, Mouse monoclonal

clone FN-15, purified from hybridoma cell culture

• Synonyme(s) : Anti-CIG, Anti-Cold-insoluble globulin, Anti-FN
• Code UNSPSC : 12352203
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: mouse
• Niveau de qualité: 200
• Forme d'anticorps: purified from hybridoma cell culture
• Type de produit anticorps: primary antibodies
• Clone: FN-15, monoclonal
• Forme: buffered aqueous solution
• Poids mol.: 220-260 kDa
• Espèces réactives: human, mouse
• Concentration: ~1 mg/mL
• Technique(s): immunoblotting: 0.3-0.6 μg/mL using human fetal fibroblast-like WI-38 cell line extract; immunofluorescence: 2.5-5 μg/mL using human foreskin fibroblast Hs68 cells; immunohistochemistry: suitable; immunoprecipitation (IP): suitable; indirect ELISA: 0.15-0.3 μg/mL using 5 μg/mL Fibronectin from human plasma for coating
• Isotype: IgG1
• Numéro d'accès UniProt: P02751
• Conditions d'expédition: dry ice
• Température de stockage: −20°C
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... FN1(2335)

Description

Description générale

Monoclonal Anti-Maltose Binding Protein (MBP) (mouse IgG1 isotype) is derived from the hybridoma MBP-17 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a purified recombinant MBP fusion protein. Fibronectin (FN), also known as Cold-insoluble globulin (CIG), is a multi-domain glycoprotein composed of two nearly identical disulfide-bound polypeptides. It is widely expressed by multiple cell types and is critically important in vertebrate development, as demonstrated by the early embryonic lethality of mice with targeted inactivation of the FN1 gene 6. In vertebrates, two types of Fibronectin are present: soluble plasma Fibronectin and insoluble cellular Fibronectin. The plasma form of Fibronectin is synthesized by hepatocytes, secreted to blood and, upon tissue injury, is incorporated into fibrin clots to exert effects on platelet function and to mediate hemostasis. Cellular Fibronectin is synthesized by many cell types, including fibroblasts, endothelial cells, chondrocytes, synovial cells and myocytes, it is assembled by cells as they migrate into the clot to reconstitute damaged tissue.

Spécificité

Anti-Fibronectin antibody, Mouse monoclonal specifically recognizes Fibronectin from plasma and in the pericellular extracellular matrix of cultured fibroblasts from human and mouse origin.

Immunogène

Fibronectin isolated from human plasma.

Application

The antibody may be used in various immunochemical techniques including Immunoblotting (∼220-260kDa)1, ELISA, Immunofluorescence, Immunohistochemistry and Immunoprecipitation.

Actions biochimiques/physiologiques

The plasma form of Fibronectin is synthesized by hepatocytes, secreted to blood and, upon tissue injury, is incorporated into fibrin clots to exert effects on platelet function and to mediate hemostasis. Cellular Fibronectin is synthesized by many cell types, including fibroblasts, endothelial cells, chondrocytes, synovial cells and myocytes, it is assembled by cells as they migrate into the clot to reconstitute damaged tissue.

Forme physique

Supplied as a solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide as a preservative.

Autres remarques

In order to obtain best results in different techniques and preparations we recommend determining optimal working concentration by titration test.

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable