S9200

Anti-SORL1 (C-terminal) antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution

• Synonyme(s) : Anti-LDLR class A repeats containing, Anti-LR11, Anti-LRP9, Anti-SORLA, Anti-SorLA-1, Anti-Sortilin-related receptor, Anti-gp250
• Code UNSPSC : 12352203
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: rabbit
• Conjugué: unconjugated
• Forme d'anticorps: affinity isolated antibody
• Type de produit anticorps: primary antibodies
• Clone: polyclonal
• Forme: buffered aqueous solution
• Poids mol.: antigen ~250 kDa
• Espèces réactives: rat, human
• Conditionnement: antibody small pack of 25 μL
• Concentration: ~1.0 mg/mL
• Technique(s): western blot: 1-2 μg/mL using rat cerebellum (S1 fraction)
• Numéro d'accès UniProt: Q92673
• Conditions d'expédition: dry ice
• Température de stockage: −20°C
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... SORL1(6653) ; mouse ... Sorl1(20660) ; rat ... Sorl1(300652)

Description

Description générale

Sortilin related receptor 1 (SORL1), a multifunctional protein, is also known as SorLA-1, LR11, LRP9 and gp250. It is a type-I transmembrane protein highly expressed in neurons. This protein belongs to the low-density lipoprotein receptor family. It contains a transmembrane domain, a large extracellular domain and a short cytoplasmic tail. SORL1 gene is located on human chromosome 11q 23/24. This mosaic receptor contains a domain that shares similarity with the vacuolar protein sorting 10 (VPS10) and with the low-density lipoprotein receptor (LDLR) family of proteins.

Spécificité

Anti-SORL1 (C-terminal) specifically recognizes rat SORL1.

Application

Anti-SORL1 (C-terminal) antibody produced in rabbit has been used in immunoblotting.

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)

Actions biochimiques/physiologiques

Sortilin related receptor 1 (SORL1) protein plays a key role in intracellular protein transport and endocytosis, cell signaling and the proliferation and migration of smooth muscle cells. It acts as a receptor for lipoproteins, glial cell-line-derived neurotrophic factor (GDNF) and platelet-derived growth factor (PDGF). Expression of this protein is developmentally regulated, with high transcript levels found during morphogenesis. SORLA is involved in development of atherosclerosis and in Alzheimer′s disease (AD). It mediates the uptake of apolipoprotein E (ApoE)-rich lipoproteins and is markedly induced in atheroma. SORL1 is known to play a regulating role in trafficking and processing of the amyloid precursor protein (APP) in neuronal cells. In the Golgi, SorLA/LR11 binding of APP leads to sequestration of APP and thereby it blocks the processing to the beta-amyloid peptide.

Forme physique

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Stockage et stabilité

Store at –20 °C. For continuous use, the product may be stored at 2–8 °C for up to one month. For extended storage, freeze in working aliquots at –20 °C. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Clause de non-responsabilité

Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 1