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RAB0366

Sigma-Aldrich

Mouse MMP-2 ELISA Kit

for serum, plasma and cell culture supernatant

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About This Item

Code UNSPSC :
41116158
Nomenclature NACRES :
NA.32

Espèces réactives

mouse

Conditionnement

kit of 96 wells (12 strips x 8 wells)

Technique(s)

ELISA: suitable
capture ELISA: suitable

Entrée

sample type plasma
sample type serum
sample type cell culture supernatant(s)

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 25 pg/mL
standard curve range: 27.43-20000 pg/mL

Méthode de détection

colorimetric

Conditions d'expédition

wet ice

Température de stockage

−20°C

Informations sur le gène

mouse ... Mmp2(17390)

Description générale

Matrix Metallopeptidase 2 (MMP-2), also known as gelatinase A, belongs to the gelatinase subclass of the matrix metalloproteinase (MMP) family. It contains zinc in the active site. MMP-2 cleaves gelatin, type IV, V, VII, X, and XI collagens, fibronectin, vitronectin, elastin, laminin, proteoglycans, and a range of non-extracellular matrix (ECM) components. Both MMP-2 and MMP9 are important for matrix degradation and they are implicated in the maintenance of neovascularization. The Mouse MMP-2 enzyme-linked immunosorbent assay (ELISA) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse MMP-2 in serum, plasma, cell culture supernatants, and urine.

Immunogène

Recombinant Mouse MMP2

Application

For research use only. Not for use in diagnostic procedures.
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)
Mouse MMP-2 ELISA Kit has been used in enzyme-linked immunosorbent assay to quantify matrix metalloproteinase -2 (MMP-2) secreted in supernatants by gold nanoparticles (AuNP)-loaded endothelial cells and superparamagnetic iron oxide nanoparticle (SPION) loaded endothelial cells.

Autres remarques

A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.

Composants de kit également disponibles séparément

Réf. du produit
Description
FDS
  • RABELADEELISA 5X Assay/Sample Diluent Buffer E (Item E2)FDS
  • RABSTOP3ELISA Stop Solution (Item I)FDS
  • RABTMB3ELISA Colorimetric TMB Reagent (HRP Substrate, Item H)FDS
  • RABWASH420X Wash Buffer (Item B)FDS

Pictogrammes

Corrosion
GHS05

Mention d'avertissement

Warning

Mentions de danger

H290

Conseils de prudence

P234 - P390

Classification des risques

Met. Corr. 1

Code de la classe de stockage

8B - Non-combustible corrosive hazardous materials

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

Subhadeep Chakrabarti et al.
Experimental lung research, 31(6), 599-621 (2005-07-16)
The lung is affected by a variety of disease processes that can lead to considerable morbidity and mortality. As the lung is the only organ for respiration and gas exchange, the structural and functional integrity of the lung is of
Disturbance of adhesomes by gold nanoparticles reveals a size- and cell type-bias.
Mulens-Arias, et al.
Biomaterials Science, 7, 389-408 (2019)
Takayuki Shiomi et al.
Pathology international, 60(7), 477-496 (2010-07-03)
Cellular functions within tissues are strictly regulated by the tissue microenvironment which comprises extracellular matrix and extracellular matrix-deposited factors such as growth factors, cytokines and chemokines. These molecules are metabolized by matrix metalloproteinases (MMP), a disintegrin and metalloproteinases (ADAM) and
Theranostic iron oxide nanoparticle cargo defines extracellular vesicle-dependent modulation of macrophage activation and migratory behavior
Mulens-Arias, V, et al.
Advanced Biosystems, 1800079-1800079 (2018)
J C Rodriguez-Manzaneque et al.
Proceedings of the National Academy of Sciences of the United States of America, 98(22), 12485-12490 (2001-10-19)
Growth of tumors and metastasis are processes known to require neovascularization. To ascertain the participation of the endogenous angiogenic inhibitor thrombospondin-1 (TSP1) in tumor progression, we generated mammary tumor-prone mice that either lack, or specifically overexpress, TSP1 in the mammary
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