P5998

Monoclonal Anti-hPlk1 (Polo Like Kinase 1) antibody produced in mouse

~2 mg/mL, clone 35-206, purified immunoglobulin, buffered aqueous solution

• Numéro MDL: MFCD07370940
• Code UNSPSC : 12352203
• eCl@ss : 32160410
• Nomenclature NACRES : NA.44

Propriétés

• Source biologique: mouse
• Conjugué: unconjugated
• Forme d'anticorps: purified immunoglobulin
• Type de produit anticorps: primary antibodies
• Clone: 35-206, monoclonal
• Forme: buffered aqueous solution
• Espèces réactives: human, rat, mouse
• Concentration: ~2 mg/mL
• Technique(s): immunocytochemistry: suitable; immunoprecipitation (IP): suitable; microarray: suitable; western blot: 0.04-0.08 μg/mL using whole extract of HeLa cell line
• Isotype: IgG2b
• Numéro d'accès UniProt: P53350
• Conditions d'expédition: dry ice
• Température de stockage: −20°C
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... PLK1(5347) ; mouse ... Plk1(18817) ; rat ... Plk1(25515)

Description

Description générale

Monoclonal Anti- hPlk1 (Polo Like Kinase 1) (mouse IgG2b isotype) is derived from the 35-206 hybridoma produced by the fusion of mouse myeloma cells (PAI) and splenocytes from BALB/c mice immunized with human full length Plk1. Polo-like kinases (Plks) are a family of serine/threonine kinases. The Plk family is structurally and functionally related to the polo gene in Drosophila. Plk1 localizes during mitosis in centrosomes, kinetochores and in the central spindle.

Immunogène

human full length Plk1.

Application

Monoclonal Anti-hPlk1 (Polo Like Kinase 1) antibody produced in mouse has been used in:

• enzyme linked immunosorbent assay (ELISA)
• immunoblotting
• immunoprecipitation
• immunocytochemistry
• immunofluorescence staining

Actions biochimiques/physiologiques

PLK1 is a serine-threonine kinase that is expressed in mitotic cells and cancers. This kinase regulates mitotic entry by modulating cdc2/cyclin B and is also involved in Nlp-mediated nucleation of microtubules .

Polo Like Kinase 1 (Plk1) has a role in tumor progression. Plk1 is a target of the DNA damage checkpoint on the basis that its activity is inhibited by DNA damage in G2 and mitosis. Moreover, Plk1 was found to bind to the sequence-specific DNA binding domain of p53. This interaction between p53 and Plk1 inhibits the transcriptional activation and the proapoptotic functions of p53.

Forme physique

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)