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N1665

Sigma-Aldrich

5′-Nucleotidase human

recombinant, expressed in CHO cells, vial of 6-12 μg

Synonyme(s) :

CD73, NT5E, ecto-5′-nucleotidase

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About This Item

Numéro CAS:
9027-73-0
Numéro de classification (Commission des enzymes):
3.1.3.5 (BRENDA, IUBMB)
Numéro MDL:
MFCD00130311
Code UNSPSC :
12352204
Nomenclature NACRES :
NA.54

Produit recombinant

expressed in CHO cells

Niveau de qualité

200

Description

contains a C-terminal 6-His tag

Pureté

≥90% (SDS-PAGE)

Forme

solution

enzyme activity

≥15 units/mg protein

Poids mol.

~61 kDa by SDS-PAGE (reducing)

Conditionnement

vial of 6-12 μg

Impuretés

≤1.0 EU/μg endotoxin

Numéro d'accès NCBI

NP_002517

Conditions d'expédition

dry ice

Température de stockage

−70°C

Informations sur le gène

human ... NT5E(4907)

Description générale

5′-Nucleotidase is an extracellular enzyme found in a wide variety of mammalian cells. It helps to accelerate the hydrolysis reaction of phosphate group from 5′ nucleotides, resulting in corresponding nucleosides.

Actions biochimiques/physiologiques

5′-Nucleotidase has various clinical significances. It is a key molecule in the regulation of cancer cells proliferation, migration and invasion in vitro tumor angiogenesis, and tumor immune escape in vivo. Due to this important role, the enzyme is a potential target for cancer research. It is also involved in salvage of extracellular nucleotides and plays a key role in the control of tissue homeostasis.
5′-nucleotidase, also known as CD73, is an extracellular enzyme that converts nucleoside-5′−monophosphates to nucleosides with a substrate preference of AMP. Native 5′-nucleotidase is a GPI-anchored protein whose exporession is upregulated by hypoxia. 5′-nucleotidase has many functions in vivo including the generation of extracellular adenosine.

Définition de l'unité

One unit will hydrolyze 1.0 μmole of inorganic phosphorus from adenosine 5′-monophosphate per min at pH 9.0 at 37 °C.

Forme physique

Supplied as a solution containing Tris, NaCl, CaCl2, and 20% glycerol.

Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

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Zhao-wei Gao et al.
BioMed research international, 2014, 460654-460654 (2014-08-16)
Purinergic signaling has emerged as an important player in cancer progression and is regulated by a series of nucleotidases. Among the enzyme cascade, CD73, which catelyzes AMP breakdown to adenosine, has been found to be overexpressed in many types of
Anna Wilk et al.
Scientific reports, 10(1), 651-651 (2020-01-22)
Changes in nicotinamide adenine dinucleotide (NAD+) levels that compromise mitochondrial function trigger release of DNA damaging reactive oxygen species. NAD+ levels also affect DNA repair capacity as NAD+ is a substrate for PARP-enzymes (mono/poly-ADP-ribosylation) and sirtuins (deacetylation). The ecto-5'-nucleotidase CD73
Isolation of a plasma-membrane subfraction from rat liver containing an insulin-sensitive cyclic-AMP phosphodiesterase.
P D House et al.
European journal of biochemistry, 24(3), 429-437 (1972-01-21)
5'-Nucleotidase: molecular structure and functional aspects.
H Zimmermann
The Biochemical journal, 285 ( Pt 2), 345-365 (1992-07-15)
Francis Coffey et al.
The Journal of experimental medicine, 211(2), 329-343 (2014-02-05)
Numerous studies indicate that γδ T cell receptor (γδTCR) expression alone does not reliably mark commitment of early thymic progenitors to the γδ fate. This raises the possibility that the γδTCR is unable to intrinsically specify fate and instead requires
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