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MAK313

Sigma-Aldrich

Adipolysis Assay Kit

sufficient for 200 colorimetric or fluorometric tests

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About This Item

Code UNSPSC :
12161503
Nomenclature NACRES :
NA.84

Application(s)

pharmaceutical

Méthode de détection

colorimetric
fluorometric

Maladie(s) pertinente(s)

endocrinological disorders, diabetes; obesity; cardiovascular diseases; cancer

Température de stockage

−20°C

Description générale

Obesity is a chronic condition that develops from storage of excessive energy in the form of adipose tissue. The resulting adiposity presents a high risk factor for diseases such as type 2 diabetes, cardiovascular diseases, and cancer. Adipolysis or lipolysis is a highly regulated process in fat metabolism, in which triglycerides are broken down into glycerol and free fatty acids. Rapid, robust and accurate procedures for adipolysis quantification in cell culture are very useful in research and drug discovery.

Caractéristiques et avantages

Compatible with high-throughput handling systems.

Adéquation

Suitable for adipolysis quantification in cell culture.

Principe

This adipolysis assay kit directly measures glycerol released during adipolysis in cultured cells. This homogeneous assay uses a single Working Reagent that combines glycerol kinase, glycerol phosphate oxidase, and color reactions in one step, resulting in a colorimetric (570 nm)/fluorimetric (λex = 530/ λem = 587 nm) product, proportional to the glycerol present.

Pictogrammes

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Mention d'avertissement

Warning

Mentions de danger

Classification des risques

Acute Tox. 4 Oral

Code de la classe de stockage

10 - Combustible liquids

Point d'éclair (°F)

188.6 °F

Point d'éclair (°C)

87 °C


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