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MAK118

Sigma-Aldrich

Invertase Activity Assay Kit

sufficient for 100 colorimetric or fluorometric tests

Synonyme(s) :

β-fructofuranosidase Assay Kit

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About This Item

Code UNSPSC :
12161503
Nomenclature NACRES :
NA.84

Utilisation

sufficient for 100 colorimetric or fluorometric tests

Application(s)

cosmetics
environmental
food and beverages

Méthode de détection

colorimetric
fluorometric

Température de stockage

−20°C

Description générale

Invertase (β-fructofuranosidase) is an enzyme that catalyzes the hydrolysis of sucrose to fructose and glucose. Invertase is produced by certain plants, microorganisms, and honey bees.

Application

Invertase Assay Kit has been used for screening inhibition of recombinant invertase inhibitor protein from sugarcane.

Caractéristiques et avantages

Compatible with high-throughput handling systems.

Adéquation

Suitable for the detection of invertase activity in biological and environmental samples and for the screening of invertase inhibitors.

Principe

In this kit, invertase activity is determined by a coupled enzyme assay in which invertase cleaves sucrose to glucose and fructose, resulting in a colorimetric (570 nm)/fluorometric (λex = 535/λem = 587 nm) product, proportional to the invertase activity present. One unit of Invertase is the amount of enzyme that will catalyze the formation of 1.0 μmole of glucose per minute at pH 4.5 under the assay conditions.

Code de la classe de stockage

10 - Combustible liquids


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