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M0643

Sigma-Aldrich

Milieu essentiel minimum d′Eagle

With Earle′s salts, L-glutamine, and non-essential amino acids, without sodium bicarbonate, powder, suitable for cell culture

Synonyme(s) :

MEM

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About This Item

Code UNSPSC :
12352207
Nomenclature NACRES :
NA.75

Niveau de qualité

500

Forme

powder

Technique(s)

cell culture | mammalian: suitable

Composants

HEPES: no
Earle’s salts (5% CO2): yes
NaHCO3: no
phenol red: yes
L-glutamine: yes
sodium pyruvate: no

Conditions d'expédition

ambient

Température de stockage

2-8°C

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Description générale

It contains 19 amino acids. The essential amino acids and the non-essential amino acids; L-ala; L-asn; L-asp; L-glu; L-gly; L-pro and L-ser. May be preferred for growth of adherent cells in the presence of reduced serum or as a base for development of a serum free medium.
Minimum Essential Medium Eagle consists of inorganic salts, water-soluble vitamins, sugar, and essential amino acids. It is widely used as a media formulation for many cell culture studies. It is a modified version of Basal medium Eagle (BME) and the concentrations of the majority of amino acids are twice as compared to BME.

Application

Minimum Essential Medium Eagle has been used to culture osteoblast-like cell line MG-63, dispersed ventricular cells, and endometrial adenocarcinoma Ishikawa cells.

Quantité

Formulated to contain 9.6 grams of powder per liter of medium.

Reconstitution

Supplement with 2.2 g/L sodium bicarbonate.

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Description
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Supplément

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Description
Tarif

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 2

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

Tatsuma Yao et al.
Reproductive medicine and biology, 16(2), 99-117 (2017-12-21)
Cell culture technology has spread prolifically within a century, a variety of culture media has been designed. This review goes through the history, characteristics and current issues of animal-cell culture media. A literature search was performed on PubMed and Google
János Magyar et al.
European journal of pharmacology, 487(1-3), 29-36 (2004-03-23)
Concentration-dependent (10-1000 microM) effects of terpenoid phenol derivatives were studied on L-type Ca(2+) current in isolated canine and human ventricular cardiomyocytes using the whole-cell configuration of patch clamp technique. Carvacrol, thymol and eugenol suppressed peak Ca(2+) current at +5 mV
Atomic layer deposited TiO2 films on an equiatomic NiTi shape memory alloy for biomedical applications
Abbas A, et al.
Journal of alloys and compounds, 161282-161282 (2021)
Sarah M Davis et al.
Molecular therapy. Nucleic acids, 21, 266-277 (2020-07-02)
Small interfering RNAs (siRNAs) have the potential to treat a broad range of diseases. siRNAs need to be extensively chemically modified to improve their bioavailability, safety, and stability in vivo. However, chemical modifications variably impact target silencing for different siRNA sequences
Tatsuma Yao et al.
Reproductive medicine and biology, 16(2), 99-117 (2017-12-21)
Cell culture technology has spread prolifically within a century, a variety of culture media has been designed. This review goes through the history, characteristics and current issues of animal-cell culture media. A literature search was performed on PubMed and Google
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Articles

Water for Mammalian Cell Culture

Ensure optimal cell culture outcomes with ultrapure, endotoxin-free water. Learn about the impact of water impurities on mammalian cell cultures and how to achieve optimal cell growth.

Water for Mammalian Cell Culture

Ensure optimal cell culture outcomes with ultrapure, endotoxin-free water. Learn about the impact of water impurities on mammalian cell cultures and how to achieve optimal cell growth.

Water for Mammalian Cell Culture

Ensure optimal cell culture outcomes with ultrapure, endotoxin-free water. Learn about the impact of water impurities on mammalian cell cultures and how to achieve optimal cell growth.

Water for Mammalian Cell Culture

Ensure optimal cell culture outcomes with ultrapure, endotoxin-free water. Learn about the impact of water impurities on mammalian cell cultures and how to achieve optimal cell growth.

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Protocoles

Powdered Media Preparation Instructions

Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.

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