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Key Documents

L8146

Sigma-Aldrich

Lectin from Ulex europaeus (gorse, furze)

peroxidase conjugate, lyophilized powder

Synonyme(s) :

Ulex europaeus agglutinin, UEA-I

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About This Item

Code UNSPSC :
12352202
Nomenclature NACRES :
NA.32

Source biologique

Ulex europaeus

Niveau de qualité

200

Conjugué

peroxidase conjugate

Pureté

70.00-90.00% (Lowry)

Forme

lyophilized powder

Puissance

<40 μg/mL agglutination activity

peroxidase activity

≥15 units/mg protein

Composition

Protein, ~80% Lowry

Technique(s)

western blot: suitable

Température de stockage

2-8°C

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Application

General Western Blot Protocol:
  • Glycoprotein sample size: 500ng
  • Lectin Concentration: 0.1ug/ml

  1. Load samples at 500 ng of glycoprotein per lane
  2. Run 4-20% Bis-Tris SDS page gel
  3. Transfer gel to a PVDF membrane
  4. Block membrane for 1 hr at RT with RIPA buffer (R0278 Sigma)
  5. Incubate HRP lectin at 0.1ug/ml with RIPA buffer for 2 hours at RT
  6. Wash membrane 5 x 5 minutes with 25ml RIPA buffer
  7. Detect using chemiluminescent substrate (CPS1-120)

Actions biochimiques/physiologiques

Ulex europaeus agglutinin has anti-H blood group specificity. There are two types of lectin: UEA I has an affinity for L-fucose and UEA II has an affinity for N,N′-diacetylchitobiose. The mol. wt. of UEA I was initially found to be 170,000 daltons. Later reports indicate that UEA I may form aggregates at neutral and basic pH and that the correct mol. wt. is 68,000 daltons.

Conditionnement

Package size based on protein content.

Liaison

Conjugates are prepared from affinity purified lectin.

Définition de l'unité

One unit will form 1.0 mg purpurogallin in 20 sec from pyrogallol at pH 6.0 at 20 °C.

Forme physique

Contains sodium citrate buffer salts

Notes préparatoires

Prepared from peroxidase, Type VI (P8375), using the two-step glutaraldehyde method of Avrameas and Ternyck.
Repurified by affinity chromatography after conjugation.

Remarque sur l'analyse

Agglutination activity is expressed in μg/ml and is determined from serial dilutions in phosphate buffered saline, pH 6.8, of a 1 mg/ml solution. This activity is the lowest concentration to agglutinate a 2% suspension of human blood group O erythrocytes after 1 hour incubation at 25 °C.

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, type N95 (US)

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Peroxidase labelled antibody and Fab conjugates with enhanced intracellular penetration.
S Avrameas et al.
Immunochemistry, 8(12), 1175-1179 (1971-12-01)
F Bucardo et al.
Zoonoses and public health, 63(8), 600-607 (2016-05-14)
Information about porcine norovirus (PoNoV), genetically similar to human NoV (HuNoV), is limited from rural areas where household-raised pigs are heavily exposed to faecal material which could facilitate transmission. Histo-blood group antigens (HBGAs) are known susceptibility factors to NoV in
Nadine Mikuda et al.
The Journal of pathology, 251(2), 160-174 (2020-03-30)
The IκB kinase (IKK)-NF-κB signaling pathway plays a multifaceted role in inflammatory bowel disease (IBD): on the one hand, it protects from apoptosis; on the other, it activates transcription of numerous inflammatory cytokines and chemokines. Although several murine models of
Xiaohu Fan et al.
Human gene therapy, 21(7), 877-890 (2010-02-19)
The ABO histo-blood group system is the most important antigen system in transplantation medicine, yet no small animal model of the ABO system exists. To determine the feasibility of developing a murine model, we previously subcloned the human alpha-1,2-fucosyltransferase (H-transferase
K Nozaki et al.
Journal of neurology, neurosurgery, and psychiatry, 80(8), 904-908 (2009-02-26)
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