KMM-201NV

KOD One^™ PCR Master Mix -BLUE-

Ready-to-use 2X hot-start PCR master mix with a modified KOD DNA polymerase optimized for ultra-fast and convenient high-fidelity PCR

• Synonyme(s) : Fast Hot-start PCR, High-fidelity DNA polymerase, KOD One^™ PCR Ready Mix
• Code UNSPSC : 41106314
• Nomenclature NACRES : NA.55

Propriétés

• Source biologique: Escherichia coli (carrying the cloned UKOD DNA polymerase gene)
• Niveau de qualité: 200
• Forme: liquid
• Utilisation: sufficient for 25 reactions; sufficient for 500 reactions
• Caractéristiques: Fast PCR; High Fidelity PCR; dNTPs included; hotstart
• Technique(s): PCR: suitable
• Entrée: crude DNA
• Conditions d'expédition: dry ice
• Température de stockage: −20°C

Description

Description générale

KOD One^™ PCR master Mix -Blue- is a ready-to-use 2 x PCR master mix containing a novel genetically modified KOD DNA polymerase (UKOD) along with a new elongation accelerator, enabling fast PCR with an extension time of 5 sec/ kb for template DNA <10kb. This master mix has greater efficiency and flexibility than conventional PCR enzymes with an ability to amplify from crude specimens such as blood and mouse cell lysates. In addition to crude samples, it can be used on templates containing uracils (dU). The master mix can use primers containing both inosines (dI) and dU for amplification.

This hot start KOD polymerase is antibody-inactivated with two types of antibodies that prevent the 5′→3′ polymerase activity and 3′→5′ exonuclease activity. Upon heat-activation, the 3′→5′ proof-reading ability of the enzyme generates blunt-end PCR products.

Application

• Genomic DNA amplification
• cDNA amplification
• Direct PCR
• Colony PCR
• Amplification of NGS libraries
• Site direct gene mutation

Compatible Sample Types: Serum, Plasma, Cell, Plant, soil extraction, DNA, nail, hair etc.
Sample Volume Needed: about 50ng DNA

Caractéristiques et avantages

• Fast: Amplifies the targets using the following very short conditions:

o =1 kb: 1 sec
o 1∼ 10 kb: 5 sec/ kb
o 10 kb∼: 10 sec/ kb

• Ready-to-use: 2X premixed solution of UKOD DNA polymerase, buffer, dNTPs, and MgCl₂. KOD One PCR Master Mix Blue includes a loading dye (BPB) to allow direct loading onto agarose gels.
• High Fidelity: Approximately 80-fold higher fidelity than conventional Taq DNA polymerase. This mix can be used for various purposes where this would be an advantage (e.g., in the preparation of long target amplicons for sequencing).
• High Efficiency: Enables high throughput PCR and ultra-fast PCR cycling conditions with an extension time of as quick as 5 sec/kb.
• Crude Sample PCR: Effective for direct PCR amplification of crude samples such as biological samples, food samples, soil extract, etc.
• High flexibility: Amplifies templates containing uracils (dU). Also, amplifies fragments using degenerate primers with inosines (dI) and uracils (dU)

Conditionnement

KMM-201NV - 5X1ML is sufficient for 500 reactions at 20 μL each or for 200 reactions at 50 μL each.
KMM-201NVS - 0.25ML is sufficient for 25 reactions at 20 μL each or for 10 reactions at 50 μL each.

Stockage et stabilité

Stored at -20 °C for a year. 4 °C for a month.

Autres remarques

For R&D use only. Not for drug, household, or other uses.

Informations légales

*Manufactured by Toyobo and distributed by Sigma-Aldrich. Not available in Japan.

KOD One is a trademark of Toyobo Co., Ltd.

Informations sur la sécurité

• Pictogrammes: GHS05
• Mention d'avertissement: Warning
• Mentions de danger: H290
• Conseils de prudence: P234 - P390
• Classification des risques: Met. Corr. 1
• Code de la classe de stockage: 8B - Non-combustible corrosive hazardous materials
• Classe de danger pour l'eau (WGK): WGK 2
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable