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I2911

Sigma-Aldrich

IMDM

StableCell™, with stable glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture

Synonyme(s) :

IMDM

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About This Item

Numéro MDL:
MFCD00217469
Code UNSPSC :
12352207
Nomenclature NACRES :
NA.75

product name

Milieu de Dulbecco modifié par Iscove, With stable glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture

Stérilité

sterile-filtered

Forme

liquid

Technique(s)

cell culture | hybridoma: suitable
cell culture | mammalian: suitable

Impuretés

Endotoxin, tested

Composants

NaHCO3: yes
glucose: yes
stable glutamine: yes
phenol red: yes

Conditions d'expédition

ambient

Température de stockage

2-8°C

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Description générale

This IMDM differs from the original formula, because it is supplemented with L-alanyl-L-glutamine dipeptide. Glutamine is notoriously unstable in cell culture. This dipeptide provides a more stable form of glutamine for use in cell cultures.

Application

Guilbert and Iscove demonstrated that precursor cells of erythrocytes and macrophages could be cultured in a totally defined serum free medium supplemented with albumin, transferrin, lecithin, and selenium. This medium is a modification of Dulbecco′s Modified Eagle′s Medium (DME) containing selenium, additional amino acids and vitamins, sodium pyruvate, HEPES buffer, and potassium nitrate instead of ferric nitrate. Further studies demonstrated that Iscove′s Medium would support murine B lymphocytes, hemopoietic tissue from bone marrow, B cells stimulated with lipopolysaccharide, T lymphocytes, and a variety of hybrid cells.

Informations légales

StableCell is a trademark of Sigma-Aldrich Co. LLC

Produit(s) apparenté(s)

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Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

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Eleni Gelali et al.
Nature communications, 10(1), 1636-1636 (2019-04-11)
DNA fluorescence in situ hybridization (DNA FISH) is a powerful method to study chromosomal organization in single cells. At present, there is a lack of free resources of DNA FISH probes and probe design tools which can be readily applied.
Christine Bourgeois et al.
Proceedings of the National Academy of Sciences of the United States of America, 105(25), 8691-8696 (2008-06-20)
A model of chemical thymectomy by inducible Rag ablation was used to study peripheral T cell homeostasis. Induction of Rag ablation was efficient and complete, leading to cessation of thymic T cell production within 3-4 weeks. The decay of peripheral
Gustav Nilsonne et al.
Behavioral and brain functions : BBF, 7, 47-47 (2011-11-22)
Recent advances in cell biology and gene regulation suggest mechanisms whereby associative learning could be performed by single cells. Therefore, we explored a model of classical conditioning in human macrophages in vitro. In macrophage cultures, bacterial lipopolysaccharide (LPS; unconditioned stimulus)
Florian A Salomons et al.
Molecular and cellular biology, 29(7), 1774-1785 (2009-01-23)
Conditions causing an increase in misfolded or aberrant proteins can impair the activity of the ubiquitin/proteasome system (UPS). This observation is of particular interest, given the fact that proteotoxic stress is closely associated with a large variety of disorders. Although

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