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H0537

Millipore

HIS-Select® HF Nickel Affinity Gel

Synonyme(s) :

Ni-NTA HF resin

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About This Item

Code UNSPSC :
41106500
Nomenclature NACRES :
NA.56

Forme

suspension

Niveau de qualité

200

Technique(s)

affinity chromatography: suitable

Matrice

Highly cross-linked 6% Beaded Agarose

Capacité

15 mg/mL, gel binding capacity (protein)(with an approx. 30 kDa protein)

Température de stockage

2-8°C

Catégories apparentées

Description générale

HIS-Select High Flow Nickel Affinity Gel is an immobilized metal-ion affinity chromatography (IMAC) gel that is designed to specifically bind His-tag proteins (histidine containing proteins) in chromatographic systems with pressures up to 200 psi and a maximum linear flow rate of 3,000 cm/hr.

Application

Affinity gel is suitable for the purification of histidine tagged proteins (His-tag proteins) in native and denaturing conditions.
Nickel Affinity Gel for the purification of His-tag® proteins (histidine tagged proteins). HIS-Select® High Flow (HF) brings the superior selectivity of HIS-Select technology to a highly cross-linked agarose for higher flow rates and mechanical stability under pressure. HIS-Select HF is designed for production scale purification and FPLC™ applications. As with other HIS-Select products, the non-charged, hydrophilic linkage of the nickel-nitrilotriacetic acid (Ni-NTA) chelate group to the agarose ensures true one-step purification.

Caractéristiques et avantages

  • High selectivity for higher purity
  • Unique non-charged hydrophilic linkage reduced non-specific binding
  • Binding Capacity for histidine-tagged protein (His-tag protein) is greater than 15 mg/mL
  • Highly cross-linked agarose matrix allows for purification under higher pressures
  • Binding under denaturing or non-denaturing conditions
  • One-step purification

Forme physique

1:1 suspension in a 30% ethanol solution

Notes préparatoires

Ethanol must be removed prior to use. Wash affinity gel in deionized water to remove the ethanol, then equilibrate with equilibration buffer that is provided.

Informations légales

FPLC is a trademark of Cytiva
HIS TAG is a registered trademark of Merck KGaA, Darmstadt, Germany
HIS-Select is a registered trademark of Merck KGaA, Darmstadt, Germany

Mention d'avertissement

Danger

Classification des risques

Acute Tox. 4 Oral - Aquatic Chronic 2 - Carc. 1A Inhalation - Flam. Liq. 3 - Muta. 2 - Repr. 1B - Resp. Sens. 1 - Skin Irrit. 2 - Skin Sens. 1 - STOT RE 1 - STOT SE 2

Organes cibles

Eyes,Central nervous system, Respiratory Tract

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

88.0 °F

Point d'éclair (°C)

31.1 °C

Équipement de protection individuelle

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

Obtaining of Agr2 Specific Antibodies and Determination of the Agr2 Protein Distribution Pattern during Early Embryonic Development and Tadpole Regeneration in Xenopus laevis
Ivanova, A.S. et al.
Russian journal of developmental biology, 49, 393-397 (2018)
Jian Ming Khor et al.
Development (Cambridge, England), 146(16) (2019-07-25)
Alx1 is a conserved regulator of skeletogenesis in echinoderms and evolutionary changes in Alx1 sequence and expression have played a pivotal role in modifying programs of skeletogenesis within the phylum. Alx1 regulates a large suite of effector genes that control
Irving E Vega et al.
Frontiers in neuroscience, 12, 879-879 (2018-12-19)
Aggregation of the protein tau is a pathological hallmark of Alzheimer's disease (AD) and related disorders. However, the molecular mechanisms that lead to tau protein aggregation are still unclear. Previously, we showed that EFhd2 protein is associated with pathological aggregated
David S Pitcher et al.
EBioMedicine, 2(7), 642-648 (2015-08-20)
The proteasome inhibitor Bortezomib is used to treat multiple myeloma (MM). Bortezomib inhibits protein degradation by inactivating proteasomes' active-sites. MM cells are exquisitely sensitive to Bortezomib - exhibiting a low-nanomolar IC(50) - suggesting that minimal inhibition of degradation suffices to
Irving E Vega et al.
Frontiers in neuroscience, 13, 845-845 (2019-08-29)
The transition of tau proteins from its soluble physiological conformation to the pathological aggregate forms found in Alzheimer's disease and related dementias, is poorly understood. Therefore, understanding the process that modulates the formation of toxic tau oligomers and their conversion
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Contenu apparenté

Technique de pull-down (interactions protéines/protéines)

Tests, réactifs et protocoles permettant d'étudier les interactions protéines/protéines in vitro par différentes méthodes : pull-down ou GST pull-down, purification par affinité en tandem (TAP pour "Tandem Affinity Purification") et co-immunoprécipitation.

Technique de pull-down (interactions protéines/protéines)

Tests, réactifs et protocoles permettant d'étudier les interactions protéines/protéines in vitro par différentes méthodes : pull-down ou GST pull-down, purification par affinité en tandem (TAP pour "Tandem Affinity Purification") et co-immunoprécipitation.

Technique de pull-down (interactions protéines/protéines)

Tests, réactifs et protocoles permettant d'étudier les interactions protéines/protéines in vitro par différentes méthodes : pull-down ou GST pull-down, purification par affinité en tandem (TAP pour "Tandem Affinity Purification") et co-immunoprécipitation.

Protein Pull-Down Techniques

Investigate in vitro protein-protein interactions with pull-down assays, utilizing affinity, GST pull-down, TAP, and co-immunoprecipitation methods.

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