GE28-9042-69
illustra™ Plasmid Mini (50)
Cytiva 28-9042-69, pack of 1 × 50 pieces
About This Item
Produits recommandés
Conditionnement
pack of 1 × 50 pieces
Fabricant/nom de marque
Cytiva 28-9042-69
Catégories apparentées
Description générale
The extracted plasmid DNA resulting from performing a miniprep is called a "plasmid miniprep" and is often performed using a plasmid purification kit. Minipreps are used in the process of molecular cloning to analyze bacterial clones. When bacteria are lysed under alkaline conditions, both chromosomal DNA and protein are denatured, but the plasmid DNA remains stable. On addition of acetate-containing neutralization buffer the larger and less supercoiled genomic DNA and proteins precipitate, but the small bacterial DNA plasmids stay in the solution.
The typical plasmid DNA yield from a fresh 1.5 mL culture of E. coli containing a high copy number plasmid is approximately 9 μg. Instead of organic solvents, the kit contains chaotropic salts to denature protein components and promote selective binding of DNA to the novel silica membrane.
The kit contains spin columns prepacked with a novel silica membrane, suspension, lysis, and neutralization buffers containing chaotropic salts, wash and elution buffers, microcentrifuge collection tubes, a full protocol booklet, and a detachable, quick reference protocol card.
Caractéristiques et avantages
- Produces high-quality plasmid DNA with excellent reproducibility for use in cloning, restriction enzyme digestion, PCR amplification, and DNA sequencing
- Total preparation time of less than 10 min represents a 50% reduction in protocol time
- Increased ease of use provided by minimal changes in pipetting volume from one part of the protocol to the next
- Fewer centrifugation steps
- Color-coded tubes and columns simplify the protocol
- No organic solvents such as phenol chloroform
Stockage et stabilité
Remarque sur l'analyse
Informations légales
Mention d'avertissement
Warning
Mentions de danger
Conseils de prudence
Code de la classe de stockage
8B - Non-combustible corrosive hazardous materials
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Articles
This page discusses methods for plasmid DNA purification and the factors impacting resulting DNA purity and yield using illustra plasmidPrep Kits from Cytiva.
This page shows possible causes and solutions for problems that may occur during plasmid DNA preparation.
Protocoles
This page shows Plasmid DNA purification using illustra™ plasmidPrep Mini Spin Kit from Cytiva.
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