Accéder au contenu
Merck
Toutes les photos(1)

Key Documents

GE17-0921-08

IMAC Sepharose 6 Fast Flow

Cytiva 17-0921-08, pack of 100 mL

Se connecterpour consulter vos tarifs contractuels et ceux de votre entreprise/organisme


About This Item

Code UNSPSC :
41106500
Nomenclature NACRES :
NA.56

Conditionnement

pack of 100 mL

Fabricant/nom de marque

Cytiva 17-0921-08

Matrice

6% cross-linked agarose

Taille des particules

45-165 μm

Diamètre moyen

90 μm

cleaning in place

2-14(Medium uncharged with metal ions.)

working range

3-12

Capacité

~40 mg binding capacity (histidine-tagged protein/ml medium when changed with Ni2+)(Protein- and metal-ion dependent.)

Adéquation

suitable for bioprocess medium

Description générale

IMAC Sepharose 6 Fast Flow is a BioProcess IMAC medium, designed for purifying proteins and peptides that have an affinity for metal ions, such as histidine-tagged proteins but also untagged recombinant or native proteins.

IMAC Sepharose 6 Fast Flow is a member of the Cytiva range of IMAC chromatography media for capture and intermediate purification.

IMAC Sepharose 6 Fast Flow is composed of cross-linked 6% agarose beads modified with a novel chelating ligand immobilized to the base matrix. IMAC Sepharose 6 Fast Flow is supplied free of metal ions, allowing the user to charge it with the most appropriate metal ion for purification of a target protein.

The coupling chemistry and the novel ligand is designed to give minimal ligand leakage while providing high capacity.

IMAC Sepharose 6 Fast Flow is available in a range of different bulk pack sizes and convenient pre-packed formats for easy scale-up and process development.
As member of the BioProcess media range, IMAC Sepharose 6 Fast Flow meets industrial demands with security of supply and comprehensive technical and regulatory support.

Caractéristiques et avantages

  • Uncharged IMAC resin allowing possibility to charge with your metal ion of choice for optimized selectivity
  • Convenient purification of histidine-tagged proteins, also when Ni2+ is not the best choice of metal ion
  • As BioProcess medium it fulfills industrial demands for security of supply, robust performance and regulatory support.
  • Available in the convenient and time-saving prepacked HiTrap® and HiPrep formats.

Stockage et stabilité

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)

Remarque sur l'analyse

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Informations légales

IMAC Sepharose products, Ni Sepharose products and Fe Sepharose products
These products are sold under a license from Sigma-Aldrich under patent number EP 1276716 (Metal chelating compositions) and equivalent patents and patent applications in other countries.
HiPrep is a trademark of Cytiva
HiTrap is a registered trademark of Cytiva
Sepharose is a trademark of Cytiva

Pictogrammes

Flame

Mention d'avertissement

Warning

Mentions de danger

Code de la classe de stockage

3 - Flammable liquids


Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

Déjà en possession de ce produit ?

Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Articles

Follow established procedures for recombinant protein expression, growth, induction, and cell lysis optimization.

Follow established procedures for recombinant protein expression, growth, induction, and cell lysis optimization.

Follow established procedures for recombinant protein expression, growth, induction, and cell lysis optimization.

Follow established procedures for recombinant protein expression, growth, induction, and cell lysis optimization.

Afficher tout

Protocoles

This page shows how to purify histidine-tagged recombinant proteins from unclarified cell lysate using HisTrap FF crude from Cytiva.

This page describes efficient column packing and preparation for affinity chromatography of tagged proteins using Cytiva products.

Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..

Contacter notre Service technique