G8918

Anti-GRP78/BiP (GL-19) antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

• Synonyme(s) : Anti-78-kDa Glucose-Regulated Protein, Anti-Immunoglobulin Heavy Chain Binding Protein
• Numéro MDL: MFCD01865530
• Code UNSPSC : 12352203
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: rabbit
• Conjugué: unconjugated
• Forme d'anticorps: IgG fraction of antiserum
• Type de produit anticorps: primary antibodies
• Clone: polyclonal
• Forme: buffered aqueous solution
• Poids mol.: antigen 78 kDa
• Espèces réactives: mouse, hamster, human
• Validation améliorée: independent; Learn more about Antibody Enhanced Validation
• Technique(s): immunocytochemistry: 1:1,000 using mouse fibroblasts NIH3T3 cells; microarray: suitable; western blot: 1:3,000 using human epitheloid carcinoma HeLa whole cell extract
• Numéro d'accès UniProt: P11021
• Conditions d'expédition: dry ice
• Température de stockage: −20°C
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... HSPA5(3309) ; mouse ... Hspa5(14828)

Description

Description générale

GRP78/BiP is a Ca²⁺-binding molecular chaperone located in the endoplasmic reticulum (ER). It is a highly conserved protein.

Application

Anti-GRP78/BiP (GL-19) antibody produced in rabbit has been used in:

• western blotting
• immunocytochemistry
• immunohistochemistry

Actions biochimiques/physiologiques

GRP78 (78-kDa glucose-regulated protein) is essential for cell viability. GRP78/BiP is induced by stress-inducing agents or by conditions that adversely affect endoplasmic reticulum(ER) function, including oxidative stress, chemical toxicity, Ca²⁺ ionophores, inhibition of the ER Ca²⁺ -ATPase, inhibitors of glycosylation and hypoxia. GRP78/BiP is critical for maintenance of Ca²⁺ homeostasis and the prevention of apoptosis. GRP78/BiP levels are reduced in Alzheimer′s disease brains and the decreased expression of GRP78/BiP is found associated with mis-sense mutations in presenilin-1 (PS-1).

Forme physique

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): nwg
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable