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G5537

Sigma-Aldrich

Glyceraldehyde-3-phosphate Dehydrogenase from baker′s yeast (S. cerevisiae)

lyophilized powder, 70-140 units/mg protein

Synonyme(s) :

D-Glyceraldehyde 3-phosphate:NAD+ oxidoreductase (phosphorylating), GAPDH

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About This Item

Numéro CAS:
9001-50-7
Numéro de classification (Commission des enzymes):
1.2.1.12 (BRENDA, IUBMB)
Numéro CE :
232-609-4
Numéro MDL:
MFCD00131203
Code UNSPSC :
12352204

Forme

lyophilized powder

Activité spécifique

70-140 units/mg protein

Composition

Protein, 30-70% biuret

Activité étrangère

3-Phosphoglyceric phosphokinase ≤0.05%

Température de stockage

−20°C

Actions biochimiques/physiologiques

Glyceraldehyde-3-phosphate dehydrogenase catalyzes the conversion of glyceraldehyde-3-phosphate to 1,3-bisphosphoglycerate as part of glycolysis. It has also been shown to have roles in initiation of apoptosis, transcription activation and the shuttling of ER to Golgi vesicles<<<,New>>>.

Définition de l'unité

One unit will reduce 1.0 μmole of 3-phosphoglycerate to D-glyceraldehyde 3-phosphate per min in a coupled system with 3-phosphoglyceric phosphokinase at pH 7.6 at 25 °C.

Forme physique

Lyophilized, sulfate-free powder stabilized with trehalose, citrate, and DTT. Useful for systems requiring low sulfate.

Inhibiteur

Réf. du produit
Description
Tarif

I2512

Sodium iodoacetate, ≥98%

Code de la classe de stockage

13 - Non Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, type N95 (US)

Certificats d'analyse (COA)

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Consulter la Bibliothèque de documents

Lei Tong et al.
The Journal of biological chemistry, 284(17), 11256-11266 (2009-03-03)
Although the classical redox functions of co-enzyme NAD(+) are firmly established in metabolism, there are numerous enzymes that catalyze cleavage of NAD(+) to yield free ADP-ribose (ADPr) or related metabolites, whose functions remain largely unknown. Here we show that the
A Tarze et al.
Oncogene, 26(18), 2606-2620 (2006-10-31)
Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a pleiotropic enzyme that is overexpressed in apoptosis and in several human chronic pathologies. Here, we report that the protein accumulates in mitochondria during apoptosis, and induces the pro-apoptotic mitochondrial membrane permeabilization, a decisive event of
Bing Fang et al.
Biochimie, 95(2), 251-257 (2012-09-27)
siRNA is promising in anti-tumor therapy. The main challenge is lack of tumor-specific intracellular delivery. In this study, a 6 amino acids peptide (A1) with high affinity for vascular endothelial growth factor receptor-1 (VEGFR1) was conjugated with a cell penetrating
Tina C Albershardt et al.
Journal of immunological methods, 384(1-2), 196-199 (2012-08-14)
Normalization to a reference gene is the method of choice for quantitative PCR analysis. The stability of reference genes is critical for accurate gene expression analysis, as significant variations in reference gene expression can alter experimental results and conclusions. In
Yuki Ikeda et al.
Archives of biochemistry and biophysics, 528(2), 141-147 (2012-09-25)
Cyclooxygenase (COX)-2 is an inducible inflammatory protein whose expression is partially regulated at the post-transcriptional level. We investigated whether glyceraldehyde-3-phosphate dehydrogenase (GAPDH) binds to the AU-rich element (ARE) of COX-2 mRNA for its degradation. Knockdown of GAPDH in hepa1c1c7 cells
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