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G5080

Sigma-Aldrich

Sephadex® G-50

Fine

Synonyme(s) :

Gel filtration resin, Sephadex G-50 Fine Medium

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About This Item

Numéro CAS:
9048-71-9
Code UNSPSC :
23151817
Nomenclature NACRES :
NA.56

Technique(s)

affinity chromatography: suitable

Groupe de la matrice active

phase

Gonflement

1 g swells to 9-11 mL

Taille des billes

20-80 μm

Application(s)

life science and biopharma

Compatibilité

Cytiva

Description générale

Sephadex G-50 Fine is a widely used gel filtration resin designed for desalting and buffer exchange of biomolecules with a molecular weight greater than 30,000. Its small bead size contributes to higher efficiency in these processes. Different types of Sephadex vary in terms of cross-linking, resulting in differences in swelling and molecular fractionation range. Sephadex G-50 is one of five G-types available, ranging from G-10 for small molecules to G-75 for larger molecules. Sephadex G-50 comes in four different particle sizes, such as Coarse, Medium, Fine, and Superfine.
Sephadex® G-50 is a gel filtration medium used in affinity chromatography, protein chromatography, and gel filtration chromatography. Sephadex is a beaded gel prepared by crosslinking dextran with epichlorohydrin.
Fractionation Range (MW)
Globular Proteins: 1,500 - 30,000
Dextrans: 500 - 10,000

Application

Sephadex® G-50 has been used:
  • to remove the non-entrapped carboxyfluorescein (CF) from the liposome suspension
  • in the purification of monoclonal antibody (mAb) humanized MN-14 by centrifuged size-exclusion chromatography
  • in desalting the recombinant enzymes eluted in the fraction five and six

Sephadex® G-50 is suitable for use in:

  • the separation of low and high molecular weight molecules
  • affinity chromatography, protein chromatography, and gel filtration chromatography

Caractéristiques et avantages

  • Desalts, removes contaminants, and transfers to a new buffer in one step.
  • Suitable for DNA purification from small molecules using gel filtration.
  • Features a small bead size, resulting in shorter diffusion distances.
  • Considered a classic gel filtration resin.

  • Desalting with Sephadex is considered superior to dialysis because it saves time, has a low dilution factor, and recovers high activity even with minute amounts of sample

Autres remarques

G5080-100G′s updated product number is GE17-0042-01
G5080-500G′s updated product number is GE17-0042-02

Informations légales

Sephadex is a registered trademark of Cytiva

Remplacé(e)(s) par

Réf. du produit
Description
Tarif

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, type N95 (US)

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Relaxation of tyrosine pathway regulation underlies the evolution of betalain pigmentation in Caryophyllales
Lopez-Nieves S, et al.
The New phytologist, 217, 896-908 (2018)
L Zhang et al.
The EMBO journal, 14(2), 313-320 (1995-01-16)
Heme is a prosthetic group for numerous enzymes, cytochromes and globins, and it binds tightly, sometimes covalently, to these proteins. Interestingly, heme also potentiates binding of the yeast transcriptional activator HAP1 to DNA and inhibits mitochondrial import of the mammalian
L M Weiner et al.
Journal of immunology (Baltimore, Md. : 1950), 151(5), 2877-2886 (1993-09-01)
Bispecific monoclonal antibodies (BsmAb) with specificity for tumor Ag and effector cell trigger molecules have been shown to redirect the cytotoxicity of several peripheral blood mononuclear cell populations against relevant tumor. The BsmAb, 2B1, binds to the extracellular domain of
Clinical-scale radiolabeling of a humanized anticarcinoembryonic antigen monoclonal antibody, hMN-14, with residualizing 131I for use in radioimmunotherapy
Govindan SV, et al.
Journal of Nuclear Medicine, 46(1), 153-159 (2005)
Samuel Lopez-Nieves et al.
The New phytologist, 217(2), 896-908 (2017-10-11)
Diverse natural products are synthesized in plants by specialized metabolic enzymes, which are often lineage-specific and derived from gene duplication followed by functional divergence. However, little is known about the contribution of primary metabolism to the evolution of specialized metabolic
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