F1757

Fumarase from porcine heart

ammonium sulfate suspension, ≥300 units/mg protein (biuret)

• Synonyme(s) : L-Malate hydrolyase, Fumarate hydratase
• Numéro CAS: 9032-88-6
• Numéro de classification (Commission des enzymes): 4.2.1.2 (BRENDA, IUBMB)
• Numéro CE : 232-887-7
• Numéro MDL: MFCD00131111
• Code UNSPSC : 12352204
• Nomenclature NACRES : NA.54

Propriétés

• Forme: ammonium sulfate suspension
• Activité spécifique: ≥300 units/mg protein (biuret)
• Activité étrangère: malic dehydrogenase ≤0.1%
• Température de stockage: 2-8°C

Description

Description générale

Fumarase (FH in human) is a well-known tricarboxylic-acid-cycle enzyme found in both the cytoplasm and mitochondria of all eukaryotes.

Fumarase is mapped to human chromosome 1q43. Fumarase comprises of three domains D1, D2 and D3 and belongs to class II fumarase family.

Application

Fumarase from porcine heart has been used:

• as a positive control in isocitrate dehydrogenase and α-ketoglutarate dehydrogenase assay
• for immobilized enzyme based stability assay
• in fumarate hydratase assays in human lung carcinoma cells (A549)

Fumarase is used as a protein calibration standard in the purification of intact DNA polymerase a/primase from mouse cells.

Actions biochimiques/physiologiques

Fumarase catalyzes the reversible hydration of fumarate to malate. In its mitochondrial form, fumarate is involved in the Krebs Cycle, while the cytosolic form is involved in amino acid metabolism.

Fumarase is essential for regulation of cellular metabolism. Fumarase elicits protective function from double-strand breaks (DSBs) induced by infrared radiation. Loss of function mutations in the fumarase gene is implicated in hereditary leiomyomatosis and renal cell cancer (HLRCC)-syndrome.

Définition de l'unité

One unit will convert 1.0 μmole of L-malate to fumarate per min at pH 7.6 at 25 °C.

Forme physique

Suspension in 3.2 M (NH₄)₂SO₄, 0.05 M KH₂PO₄, pH 7.5, 0.014 M 2-mercaptoethanol

Notes préparatoires

Isolated by the method of Beeckmans, S., and Kanarek, L., Eur. J. Biochem., 78, 437 (1977). Affinity purified.

Informations sur la sécurité

• Pictogrammes: GHS07
• Mention d'avertissement: Warning
• Mentions de danger: H317
• Conseils de prudence: P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364
• Classification des risques: Skin Sens. 1
• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable