E1281

Anti-Ezrin antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

• Synonyme(s) : Anti-80K, Anti-Cytovillin, Anti-p81
• Numéro MDL: MFCD00211933
• Code UNSPSC : 12352203
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: rabbit
• Conjugué: unconjugated
• Forme d'anticorps: affinity isolated antibody
• Type de produit anticorps: primary antibodies
• Clone: polyclonal
• Forme: buffered aqueous solution
• Poids mol.: antigen ~80 kDa
• Espèces réactives: human, rat
• Technique(s): immunohistochemistry: 20-40 μg/mL; immunoprecipitation (IP): 0.5-1 μg using RIPA extract of human A431 cells; indirect immunofluorescence: 10-20 μg/mL; western blot (chemiluminescent): 0.5-1 μg/mL using whole extract of rat NRK cells
• Numéro d'accès UniProt: P15311
• Conditions d'expédition: dry ice
• Température de stockage: −20°C
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... EZR(7430) ; mouse ... Ezr(22350) ; rat ... Ezr(54319)

Description

Description générale

Ezrin is a 80kDa membrane-associated protein that belongs to ERM (Ezrin/Radixin/Moesin) family. Ezrin is found predominantly in polarized epithelial cells such as intestinal epithelial cells, gastric parietal cells, epithelial cells of the kidney proximal tubule, terminal bronchiole of the lung, and in mesothelia.

Immunogène

synthetic peptide corresponding to amino acids 479-498 of human ezrin with N-terminal added cysteine, conjugated to KLH. The corresponding sequence in rat and mouse differs by three amino acids.

Application

Anti-Ezrin antibody produced in rabbit has been used in:

• immunolocalization studies
• immunostaining
• immunofluorescence and immunohistochemistry
• immunoblot

Actions biochimiques/physiologiques

Cultured neuronal cells do express ezrin to some extent, and whole brain tissue expresses low levels of ezrin. Ezrin expression is associated with malignancy in astrocytoma, osteosarcoma, mammary and pancreatic adenocarcinomas, and in cutaneous and uveal melanomas.

Ezrin promotes cell adhesion. The NH2-terminal domain negatively regulates membrane spreading and elongation properties of ezrin COOH-terminal domain. The members of ERM family are usually involved in cross-linking of plasma membrane and actin filaments. ERM proteins are involved in cell adhesion, cell shape determination, migration, and the organization of cell surface structures. They are also involved in signal transduction and apoptosis. Ezrin has been implicated in tumor progression and metastatic spread.

Forme physique

Solution in 0.01 M phosphate buffered saline containing 15 mM sodium azide.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): nwg
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable