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CGP1

Sigma-Aldrich

Glutathione Peroxidase Cellular Activity Assay Kit

Sufficient for 100 colorimetric tests

Synonyme(s) :

Cellular glutathione peroxidase, c-GPx

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About This Item

Code UNSPSC :
12161503
Nomenclature NACRES :
NA.32

Niveau de qualité

200

Utilisation

sufficient for 100 tests

Durée de conservation

Kit is stable for 24 months when unopened.

Méthode de détection

colorimetric

Conditions d'expédition

wet ice

Température de stockage

−20°C

Description générale

The Glutathione Peroxidase Cellular Activity Assay kit is used to measure glutathione peroxidase in tissue extracts. The assay is based on the oxidation of glutathione (GSH) to oxidized glutathione (GSSG). This is catalyzed by GPx coupled to the recycling of GSSG back to GSH utilizing glutathione reductase and NADPH. The decrease in NADPH absorbance measured at 340 nm during the oxidation of NADPH to NADP is indicative of glutathione peroxidase activity since the enzyme is the rate-limiting factor of the coupled reactions.

Adéquation

Suitable for the measureent of glutathione peroxidase in tissue extracts

Principe

The Glutathione Peroxidase Cellular Activity Assay kit is used to measure glutathione peroxidase in tissue extracts. The assay is based on the oxidation of glutathione (GSH) to oxidized glutathione (GSSG). This is catalyzed by GPx coupled to the recycling of GSSG back to GSH utilizing glutathione reductase and NADPH. The decrease in NADPH absorbance measured at 340 nm during the oxidation of NADPH to NADP is indicative of glutathione peroxidase activity since the enzyme is the rate-limiting factor of the coupled reactions.

Autres remarques

This kit was tested with rabbit reticulocyte lysate and with glutathione peroxidase enzyme.

Composants de kit seuls

Réf. du produit
Description
  • tert-Butyl hydroperoxide 1 mL

Produit(s) apparenté(s)

Réf. du produit
Description
Tarif

Mention d'avertissement

Danger

Classification des risques

Acute Tox. 2 Inhalation - Acute Tox. 3 Dermal - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Flam. Liq. 3 - Muta. 2 - Org. Perox. F - Skin Corr. 1C - Skin Sens. 1 - STOT SE 3

Organes cibles

Respiratory system

Code de la classe de stockage

5.2 - Organic peroxides and self-reacting hazardous materials

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

V R Arruda et al.
Neoplasma, 43(2), 99-102 (1996-01-01)
Red cell antioxidant enzymes have been recently studied in malignant lymphomas and the results are controversial. Hairy cell leukemia is a rare chronic lymphoproliferative disorder originating probably in a pluripotent stem cell. In the present study, glutathione peroxidase (Gpx), reduced
Diversity of glutathione peroxidases.
F Ursini et al.
Methods in enzymology, 252, 38-53 (1995-01-01)
International Committee for Standardization in Haematology: recommended methods for red-cell enzyme analysis.
E Beutler et al.
British journal of haematology, 35(2), 331-340 (1977-02-01)
Roxana Oana Cojocariu et al.
Brain sciences, 10(11) (2020-11-21)
Background and Objectives: Irritable bowel syndrome (IBS) is a well-known functional gastrointestinal (GI) disorder exhibiting a wide range of symptoms due to individual variability and multifactorial etiology. Stress exposure is a major risk factor for the development of IBS. Here
I Mavelli et al.
European journal of biochemistry, 139(1), 13-18 (1984-02-15)
Red blood cells of favism patients with acute hemolytic crisis have markedly more superoxide dismutase (superoxide:superoxide oxidoreductase, EC 1.15.1.1) and less glutathione peroxidase (glutathione:hydrogenperoxide oxidoreductase, EC 1.11.1.9) than either normal controls, glucose-6-phosphate dehydrogenase-deficient subjects or favism patients outside hemolytic crisis.
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