BF-2

87032603, fish trunk, Fibroblast

• Code UNSPSC : 41106514

Propriétés

• product name: BF-2, 87032603
• Source biologique: fish trunk
• Mode de croissance: Adherent
• Caryotype: 2n = 48
• Morphologie: Fibroblast
• Produits: Not specified
• Récepteurs: Not specified
• Technique(s): cell culture | mammalian: suitable
• Conditions d'expédition: dry ice
• Température de stockage: −196°C

Description

Origine de la lignée cellulaire

Fish Bluegill fry caudal trunk

Description de la lignée cellulaire

Derived from a trypsinised suspension of pooled caudal portions of the trunk of one year old fingerlings. Will grow at temperatures from 15°C to 33°C. Cells susceptible to lymphocystis virus of fish and tadpole edema virus (TEV) of frogs.

Application

Virus studies, targets for bacterial cytotoxins

Milieu de culture

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS) (pH7.4 - 7.5).

Procédure de repiquage

Split sub-confluent cultures (70-80%) 1:2 to 1:4 using 0.25% trypsin/EDTA; 5% CO₂; 23°C. A replication capacity of up to 50 passages is expected. Fish cell lines detach easily during transit if the culture is too young. After resuscitation, split cells

Autres remarques

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