V79-HG04

87020406, hamster lung, Fibroblast-like

• Code UNSPSC : 41106514

Propriétés

• product name: V79-HG04, 87020406
• Source biologique: hamster lung
• Mode de croissance: Adherent
• Caryotype: Aneuploid
• Morphologie: Fibroblast-like
• Produits: Not specified
• Récepteurs: Not specified
• Technique(s): cell culture | mammalian: suitable
• Maladie(s) pertinente(s): cancer
• Conditions d'expédition: dry ice
• Température de stockage: −196°C

Description

Origine de la lignée cellulaire

Hamster Chinese embyro lung fibroblast transformed

Description de la lignée cellulaire

A spontaneously transformed cell line of a primary culture derived from embryonic lung fibroblasts of a Chinese hamster. Thioguanine resistant, HAT sensitive, high cloning efficiency in soft agar and used for HPRT- genotyping selection in HAT.

Application

Cell fusion studies

Milieu de culture

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Procédure de repiquage

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C.

Autres remarques

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