C3741
Sous-unité B de la toxine cholérique
peroxidase conjugate (Contains ~ 2 moles HRP/mole of CTB. ~100 μg HRP conjugated to ~45 μg CTB), lyophilized powder
Synonyme(s) :
CTB
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About This Item
Numéro MDL:
Code UNSPSC :
12352202
ID de substance PubChem :
Nomenclature NACRES :
NA.77
Produits recommandés
Conjugué
peroxidase conjugate (Contains ~ 2 moles HRP/mole of CTB. ~100 μg HRP conjugated to ~45 μg CTB)
Niveau de qualité
Forme
lyophilized powder
peroxidase activity
>100 U/mg, pH 6.0, 20 °C
Poids mol.
pentamer 57 kDa
~12 kDa
Solubilité
H2O: soluble 10 mg/mL
Température de stockage
2-8°C
Chaîne SMILES
CCOc1ccccc1C(=O)Nc2ccc(Cl)c(c2)C(F)(F)F
InChI
1S/C16H13ClF3NO2/c1-2-23-14-6-4-3-5-11(14)15(22)21-10-7-8-13(17)12(9-10)16(18,19)20/h3-9H,2H2,1H3,(H,21,22)
Clé InChI
YDXZSNHARVUYNM-UHFFFAOYSA-N
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Description générale
Cholera toxin is produced by Vibrio cholerae species, and the gene ctxB (cortexillin II) encodes the B subunit of the toxin. Cholera toxin belongs to the AB2 family of toxins. It is composed of a single A subunit of 28 kDa and five B subunits of 11 kDa.
Spécificité
HRP activity exceedes 100 pyrogallol units per mg of HRP in the CTB-HRP conjugate. Activity: 10-30 pyrogallol units per vial.
Application
Cholera toxin B subunit conjugated to peroxidase (CB-HRP) has been shown to be a more effective retrograde and transganglionic tracer for pelvic primary afferents from the urinary bladder than wheat germ agglutinin-horseradish peroxidase or isolectin B4-horseradish peroxidase. Retrograde labeling with CB-HRP has been demonstrated in sympathetic pre-ganglionic neurons as well as in cells of the fastigial nuclei. It is also an effective anterograde tracer for the study of axonal and terminal labeling in the brain stem. Cholera Toxin B subunit has been used:
- In the isolation of lipid rafts and analysis of B-cell receptor distribution, signaling and ubiquitination.
- In the staining of lipid rafts in brain tissues.
- As a transganglionic tracer to determine the central projections of the anterior ethmoidal nerve in rat.
Actions biochimiques/physiologiques
La sous-unité B de la toxine cholérique est utilisée pour le traçage de voies en recherche neurologique, tirant profit de la liaison au ganglioside GM1 et du transport rétrograde. Les cellules issues de cultures cellulaires traitées avec de la toxine cholérique ne sont pas tuées, et les tissus animaux ne deviennent pas nécrotiques.
Cholera toxin gains entry into the host cell via host′s glycolipid transport pathway. The toxin is an essential factor for the pathogenesis of cholera. The ToxR gene is responsible for the activation of the expression of the gene coding cholera toxin.
Conditionnement
Prepared and packaged using aseptic technique and sealed under vacuum.
Attention
Do not freeze.
Définition de l'unité
Unit definition: One pyrogallol unit converts 1 mg of pyrogallol to purpurogallin in 20 seconds at pH 6.0 at 20 °C.
Forme physique
Lyophilized powder containing 11-13% protein with the balance consisting of phosphate buffer and sodium chloride.
Reconstitution
Reconstitution of the vial with 100 μl of water will yield a final solution containing: 1 mg/mL HRP, 0.45 mg/ml CTB, 10 mM phosphate buffer and 150 mM NaCl. Swirl bottles gently during reconstitution. Avoid vigorous pipetting of solutions that may lead to foaming. Solutions can be sterile filtered through a 0.2 μm filter. Store the reconstituted solutions at 2-8 °C.
Mention d'avertissement
Danger
Mentions de danger
Conseils de prudence
Classification des risques
Resp. Sens. 1
Code de la classe de stockage
13 - Non Combustible Solids
Classe de danger pour l'eau (WGK)
WGK 2
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Certificats d'analyse (COA)
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