C2181
Anti-Mouse IgG (whole molecule) F(ab′)2 fragment–Cy3 antibody produced in sheep
affinity isolated antibody, buffered aqueous solution
Synonyme(s) :
Cy3 Anti-Mouse IgG, Cy3 Mouse IgG, Sheep Anti-Mouse IgG
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About This Item
Produits recommandés
Source biologique
sheep
Conjugué
CY3 conjugate
Forme d'anticorps
affinity isolated antibody
Type de produit anticorps
secondary antibodies
Clone
polyclonal
Forme
buffered aqueous solution
Espèces réactives
mouse
Technique(s)
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100
Conditions d'expédition
wet ice
Température de stockage
2-8°C
Modification post-traductionnelle de la cible
unmodified
Description générale
Immunoglobulins (Igs) belong to the immunoglobulin super-family. IgG is an abundant protein in human serum. The four classes of IgG include IgG1, IgG2, IgG3 and IgG4.. The IgG heavy chain region is mapped to human chromosome 14. Igs have two heavy (H) and two light (L) chains, held together by disulfide linkages. The heavy chain has one variable N-terminal region and three to four constant (CH1-CH4) C-terminal regions. The L chain comprises of one variable N-terminal region and a constant C-terminal region.
Application
Anti-Mouse IgG (whole molecule) F(ab′)2 fragment-Cy3 antibody produced in sheep was used for BrdU staining of frozen quail muscle cross sections at a dilution of 1:200 to analyze activated, proliferating muscle precursor cells.
Anti-Mouse IgG (whole molecule) F(ab′)2 fragment−Cy3 antibody produced in sheep has been used:
- in immunolabeling of Hela cells
- as secondary antibody in immunocytochemistry of dendritic cells
- as secondary antibody in immunofluorescence analysis of mesenchymal stem cells
- as secondary antibody in immunofluorescence staining keratinocyte cell lines
- in immunohistochemistry of articular cartilage
The product binds to all mouse Igs and is useful when trying to avoid background staining due to the presence of Fc receptors.
Actions biochimiques/physiologiques
Digestion of IgG by papain results in the generation of fragment antigen binding (Fab). Pepsin digestion of IgG results in fragment crystallizable (Fc). The Fc region of IgG antibody has enormous therapeutic potential and is exploited for the development of therapeutic antibodies.
IgG antibody subtype is the most abundant of serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defense of the neonate against infections. The coupling of Cy3 to Anti-Mouse IgG (whole molecule) F(ab′)2 fragment antibody allows for the visualization of protein by fluorescent microscopy.
Autres remarques
Antibody adsorbed with human serum proteins.
Forme physique
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.
Notes préparatoires
Adsorbed to reduce background staining with human samples.
Clause de non-responsabilité
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Code de la classe de stockage
10 - Combustible liquids
Classe de danger pour l'eau (WGK)
nwg
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Certificats d'analyse (COA)
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