AL11430

DMT-locA(bz) Phosphoramidite

configured for ABI

• Formule empirique (notation de Hill): C₄₈H₅₂N₇O₈P
• Numéro CAS: 206055-79-0
• Poids moléculaire : 885.94
• Code UNSPSC : 41116105
• Nomenclature NACRES : NA.51

Propriétés

• Source biologique: non-animal source (BSE/TSE no Risk)
• Niveau de qualité: 300
• Gamme de produits: Proligo Reagents
• Pureté: ≥98.0% (³¹P-NMR); ≥98.0% (reversed phase HPLC)
• Forme: powder
• Impuretés: ≤3 wt. % Residual Solvent Content; <0.4% Water Content (Karl Fischer); <0.5% Single unspecified Impurity (reversed phase HPLC)
• Couleur: white to off-white
• Solubilité: soluble, clear, colorless
• Absorption: <0.1 in acetonitrile at 0.2 M
• Adéquation: conforms to structure for H-NMR; conforms to structure for LC-MS
• Compatibilité: configured for ABI
• Température de stockage: −20°C

Description

Description générale

DMT-locA(bz) Phosphoramidite belongs to the group of LNA phosphoramidite. It is known as DMT-locAdenosine(N6-Benzoyl)- ß-Cyanoethylphosphoramidite. The synthesis cycle for Locked Nucleic Acid (LNA) oligonucleotides consists of the same series of reactions as the cycle that is employed for DNA monomers. However, the rate of coupling for LNA monomers is slower compared to that of DNA monomers (a coupling time of 8 minutes is recommended for LNA monomers compared to 90 seconds for DNA monomers).

With the exception of the LNA monomers, LNA synthesis is accomplished with the same reagents as DNA synthesis. LNA phosphoramidites from Merck are diluted with dry acetonitrile, except for locMeC(bz)-phosphoramidite. This phosphoramidite requires the application of a cosolvent to prevent crystallization from the solution on the synthesizer. Dichloromethane or tetrahydrofuran (THF) can be applied as co-solvents with acetonitrile to completely dissolve locMeC(bz)-phosphoramidite.

Caractéristiques et avantages

Key Features of LNA Phosphoramidites :

• LNA oligonucleotides are prepared by phosphoramidite chemistry
• Standard DNA synthesizer platforms can be employed. No change is required in the reagents commonly used for DNA synthesis
• To further enhance the hybridization characteristics of LNA, 5-methyl-cytidine is employed instead of cytidine
• LNA monomers are as soluble in acetonitrile as their DNA counterparts (except for the 5-methyl-cytidine derivative, which requires the application of 10-20%, dichloromethane as a co-solvent)
• Mixmer oligonucleotides containing LNA, DNA and/or RNA monomers can be assembled easily
• LNA oligonucleotides with predefined melting temperatures (Tm) can be designed and prepared

Autres remarques

LNA phosphoramidites from Merck are diluted with dry acetonitrile, except for locMeC(bz)-phosphoramidite. This phosphoramidite requires the application of a cosolvent to prevent crystallization from the solution on the synthesizer. Dichloromethane or tetrahydrofuran(THF) can be applied as co-solvents with acetonitrile to completely dissolve locMeC(bz)-phosphoramidite.

Informations légales

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable