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A8678

Sigma-Aldrich

Gélose

suitable for plant cell culture

Synonyme(s) :

Agar, Agar-agar

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About This Item

Formule linéaire :
(C12H18O9)n
Numéro CAS:
9002-18-0
Numéro CE :
232-658-1
Numéro MDL:
MFCD00081288
Code UNSPSC :
50405901
Nomenclature NACRES :
NA.21

Source biologique

algae (Rhodophyceae)

Forme

powder

Technique(s)

cell culture | plant: suitable

Pf

80-90 °C (1.5% in water)

Température de transition

transition temp 27-37 °C (1.5%)

Application(s)

agriculture

Température de stockage

room temp

InChI

1S/C14H24O9/c1-5-8(16)13-11(7(21-5)4-20-13)23-14-10(18)12(19-2)9(17)6(3-15)22-14/h5-18H,3-4H2,1-2H3/t5?,6-,7?,8-,9+,10-,11?,12+,13+,14?/m1/s1

Clé InChI

GYYDPBCUIJTIBM-DYOGSRDZSA-N

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Description générale

Agar or Agar-agar is one of the major components found in the cell wall of red algae. It is a polysaccharide complex, comprising about 70% agarose and 30% agaropectin.

Application

Washed agar has been used:
  • as a supplement (0.9% w/v) to Murashige and Skoog basal medium for plant growth
  • in the preparation of cocultivation medium for Agrobacterium tumefaciens mediated transformation in maize (Zea mays) hybrid Hi-II
  • as a component in the complete medium to regenerate protoplasts of Piriformospora indica
  • as a clean agar medium to culture Synechococcus WH7803 strain

Actions biochimiques/physiologiques

Agar is an ester of sulfuric acid in a linear galactan form, that makes it soluble in hot water and insoluble in cold. Washed agar contain less impurities and low level of nutrients.

Notes préparatoires

Washed

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 1

Équipement de protection individuelle

Eyeshields, Gloves, type N95 (US)

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Alga Zuccaro et al.
Fungal genetics and biology : FG & B, 46(8), 543-550 (2009-04-09)
Piriformospora indica (Basidiomycota, Sebacinales) is a root colonizing fungus which is able to increase biomass and yield of crop plants and to induce local and systemic resistance to fungal diseases and tolerance to abiotic stress. A prerequisite for the elucidation
Andrew D Millard
Methods in molecular biology (Clifton, N.J.), 501, 33-42 (2008-12-11)
Cyanophages are a group of viruses which specifically infect cyanobacteria. The cyanobacteria are predominantly aquatic phototrophic bacteria and the two dominant genera Synechococcus and Prochlorococcus contribute significantly to primary production in the oceans. Cyanophages that infect marine cyanobacteria were first
Lawrence R Griffing
Methods in molecular biology (Clifton, N.J.), 1691, 75-102 (2017-10-19)
In this chapter, approaches to the image analysis of the choreography of the plant endoplasmic reticulum (ER) labeled with fluorescent fusion proteins ("stars," if you wish) are presented. The approaches include the analyses of those parts of the ER that
Juan M Vega et al.
Plant cell reports, 27(2), 297-305 (2007-10-17)
High-frequency transformation of maize (Zea mays L.) using standard binary vectors is advantageous for functional genomics and other genetic engineering studies. Recent advances in Agrobacterium tumefaciens-mediated transformation of maize have made it possible for the public to transform maize using
Arnaud Dechesne et al.
Applied and environmental microbiology, 74(16), 5195-5200 (2008-07-01)
Water is arguably the most important constituent of microbial microhabitats due to its control of physical and physiological processes critical to microbial activity. In natural environments, bacteria often live on unsaturated surfaces, in thin (micrometric) liquid films. Nevertheless, no experimental
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