A3574

Acrylamide/Bis-acrylamide solution

30%, BioReagent, suitable for electrophoresis, 29:1

• Synonyme(s) : 30% Acrylamide/Bisacrylamide 29:1, Polyacrylamide solution
• Numéro MDL: MFCD00080848
• Code UNSPSC : 41105319
• ID de substance PubChem : 329770837
• Nomenclature NACRES : NA.25

Propriétés

• product name: Acrylamide/Bis-acrylamide, 30% solution, BioReagent, suitable for electrophoresis, 29:1
• Stérilité: 0.2 μm filtered
• Niveau de qualité: 200
• Gamme de produits: BioReagent
• Forme: liquid
• Ratio alimentaire: 29:1
• Technique(s): electrophoresis: suitable
• Adéquation: suitable for electrophoresis
• Activité étrangère: Protease, none detected
• Température de stockage: 2-8°C; 2-8°C
• Chaîne SMILES: NC(=O)C=C.C=CC(=O)NCNC(=O)C=C
• InChI: 1S/C7H10N2O2.C3H5NO/c1-3-6(10)8-5-9-7(11)4-2;1-2-3(4)5/h3-4H,1-2,5H2,(H,8,10)(H,9,11);2H,1H2,(H2,4,5)
• Clé InChI: OCQZXMCGTAWGEQ-UHFFFAOYSA-N

Description

Description générale

Polyacrylamide gels are prepared using acrylamide and bis-acrylamide, where bis-acrylamide is a cross-linking agent. On the other hand, acrylamide is a water soluble monomer which helps in forming a transparent stable insoluble gel.

Application

Acrylamide/Bis-acrylamide, 30% solution has been used as a constituent in the preparation of 4% stacking gel and 10% resolving gel during sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) gel electrophoresis. It has also been used as a precursor for polyacrylamide lid fabrication by photopatterning methods.

These 30% (w/w) acrylamide/bis-acrylamide solutions are available at the most common ratios for use in protein and nucleic acid electrophoresis. The concentration is based on the total weight of both the acrylamide and bis-acrylamide.

Notes préparatoires

Solution prepared from electrophoresis grade acrylamide and bis-acrylamide in ultra-pure water.

Informations sur la sécurité

• Pictogrammes: GHS08,GHS07
• Mention d'avertissement: Danger
• Mentions de danger: H302 + H332,H315,H317,H319,H340,H350,H361fd,H372
• Conseils de prudence: P280 - P301 + P312 - P302 + P352 - P304 + P340 + P312 - P305 + P351 + P338 - P308 + P313
• Classification des risques: Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Carc. 1B - Eye Irrit. 2 - Muta. 1B - Repr. 2 - Skin Irrit. 2 - Skin Sens. 1 - STOT RE 1 Oral
• Organes cibles: Peripheral nervous system
• Code de la classe de stockage: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• Classe de danger pour l'eau (WGK): WGK 3
• Équipement de protection individuelle: Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter