HLA002
Jurkat HLA Panel Cell Lines
Synonyme(s) :
CAR-T, Checkpoint Inhibitors, Immuno-oncology, Immunotherapy, Oncology
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About This Item
Code UNSPSC :
41106514
Produits recommandés
Source biologique
human (T Lymphocyte (blood))
Mode de croissance
suspension
Conditions d'expédition
dry ice
Température de stockage
−196°C
Description générale
The STR profile of these cell lines match that of its parental cell line - ECACC catalog number 88042803.
This product is a panel of eleven (11) genetically modified cell lines targeting MHC Class I HLA molecules in a Jurkat T lymphocyte parental cell line background. The full HLA panel consists of a Beta-2 microglobulin (β2M) knockout cell line and an additional ten (10) monoallelic HLA expression cell lines.
CompoZr® Zinc Finger Nuclease (ZFN) technology was used to generate the β2M knockout cell line, which does not express endogenous cell surface MHC Class I HLA molecules (HLA-A or HLA-B) as measured by fluorescence-activated cell sorting (FACS).
MISSION® lentivirus was used to generate the ten (10) monoallelic HLA cell lines, which express individual HLA-A or HLA-B subtypes on the cell surface via a β2M:HLA fusion protein as described in Nature Biotechnology 35, 765-772, (2017) and as demonstrated via FACS analysis.
This product is a panel of eleven (11) genetically modified cell lines targeting MHC Class I HLA molecules in a Jurkat T lymphocyte parental cell line background. The full HLA panel consists of a Beta-2 microglobulin (β2M) knockout cell line and an additional ten (10) monoallelic HLA expression cell lines.
CompoZr® Zinc Finger Nuclease (ZFN) technology was used to generate the β2M knockout cell line, which does not express endogenous cell surface MHC Class I HLA molecules (HLA-A or HLA-B) as measured by fluorescence-activated cell sorting (FACS).
MISSION® lentivirus was used to generate the ten (10) monoallelic HLA cell lines, which express individual HLA-A or HLA-B subtypes on the cell surface via a β2M:HLA fusion protein as described in Nature Biotechnology 35, 765-772, (2017) and as demonstrated via FACS analysis.
Description de la lignée cellulaire
Derived from Jurkat FHCRC. An IL-2 producing cell line, derived by incubating the cells at 41°C for 48 hours followed by a limiting dilution cloning over macrophages.
Caractéristiques et avantages
Unlike wild type Jurkat cells, which express multiple HLA subtypes, the Jurkat HLA panel cell lines express individual MHC Class I HLA subtypes. These monoallelic HLA cell lines can be used to study peptide binding and presentation in the context of individual HLA subtypes. The panel can also be used to develop cell-based assays to evaluate candidate cancer immunotherapies and identify tumor-specific neoantigens.
Composants
HLA002A-1VL: Jurkat B2M KO Cells
HLA002B-1VL: Jurkat HLA-A*02:01 Cells
HLA002C-1VL: Jurkat HLA-A*01:01 Cells
HLA002D-1VL: Jurkat HLA-A*03:01 Cells
HLA002E-1VL: Jurkat HLA-A*11:01 Cells
HLA002F-1VL: Jurkat HLA-A*24:02 Cells
HLA002G-1VL: Jurkat HLA-B*15:10 Cells
HLA002H-1VL: Jurkat HLA-B*07:02 Cells
HLA002I-1VL: Jurkat HLA-B*08:01 Cells
HLA002J-1VL: Jurkat HLA-B*35:01 Cells
HLA002K-1VL: Jurkat HLA-B*40:01 Cells
HLA002B-1VL: Jurkat HLA-A*02:01 Cells
HLA002C-1VL: Jurkat HLA-A*01:01 Cells
HLA002D-1VL: Jurkat HLA-A*03:01 Cells
HLA002E-1VL: Jurkat HLA-A*11:01 Cells
HLA002F-1VL: Jurkat HLA-A*24:02 Cells
HLA002G-1VL: Jurkat HLA-B*15:10 Cells
HLA002H-1VL: Jurkat HLA-B*07:02 Cells
HLA002I-1VL: Jurkat HLA-B*08:01 Cells
HLA002J-1VL: Jurkat HLA-B*35:01 Cells
HLA002K-1VL: Jurkat HLA-B*40:01 Cells
Qualité
Tested for Mycoplasma, bacterial and fungal content, post-freeze viability, and short terminal repeat (STR) analysis for cell line identification.
Informations légales
CompoZr is a registered trademark of Merck KGaA, Darmstadt, Germany
MISSION is a registered trademark of Merck KGaA, Darmstadt, Germany
Clause de non-responsabilité
Ce produit, destiné à la recherche scientifique, est soumis à une réglementation spécifique en France, y compris pour les activités d′importation et d′exportation (Article L 1211-1 alinéa 2 du Code de la Santé Publique). L′acheteur (c′est-à-dire l′utilisateur FINAL) est tenu d′obtenir une autorisation d′importation auprès du Ministère français de la Recherche, mentionné à l′article L1245-5-1 II du Code de la Santé Publique. En commandant ce produit, vous confirmez détenir l′autorisation d′importation requise.
Code de la classe de stockage
10 - Combustible liquids
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