C5070

3-[(3-Cholamidopropyl)diméthylammonio]-1-propanesulfonate hydrate

BioXtra, ≥98% (TLC)

• Synonyme(s) : CHAPS
• Formule empirique (notation de Hill): C₃₂H₅₈N₂O₇S · xH₂O
• Numéro CAS: 331717-45-4
• Poids moléculaire : 614.88 (anhydrous basis)
• Numéro MDL: MFCD00012116
• Code UNSPSC : 12161900
• ID de substance PubChem : 24892750
• Nomenclature NACRES : NA.21

Propriétés

• Description: zwitterionic
• Niveau de qualité: 400
• Gamme de produits: BioXtra
• Pureté: ≥98% (TLC)
• Forme: powder
• Poids mol.: 614.88 g/mol (anhydrous basis)
• Technique(s): protein purification: suitable; protein quantification: suitable
• Impuretés: ≤0.001% Phosphorus (P); ≤0.1% Insoluble matter; <0.04% DMF
• Résidus de calcination: ≤0.1%
• Pf: 157 °C (dec.) (lit.)
• Solubilité: H₂O: 0.1 M, clear, colorless
• Traces d'anions: chloride (Cl^-): ≤0.05%
• Traces de cations: Al: ≤0.0005%; Ca: ≤0.0005%; Cu: ≤0.0005%; Fe: ≤0.0005%; K: ≤0.005%; Mg: ≤0.0005%; NH₄⁺: ≤0.05%; Na: ≤0.005%; Pb: ≤0.001%; Zn: ≤0.0005%
• Chaîne SMILES: O.C[C@H](CCC(=O)NCCC[N+](C)(C)CCCS([O-])(=O)=O)[C@H]1CC[C@H]2[C@@H]3[C@H](O)C[C@@H]4C[C@H](O)CC[C@]4(C)[C@H]3C[C@H](O)[C@]12C
• InChI: 1S/C32H58N2O7S.H2O/c1-21(8-11-29(38)33-14-6-15-34(4,5)16-7-17-42(39,40)41)24-9-10-25-30-26(20-28(37)32(24,25)3)31(2)13-12-23(35)18-22(31)19-27(30)36;/h21-28,30,35-37H,6-20H2,1-5H3,(H-,33,38,39,40,41);1H2/t21-,22+,23-,24-,25+,26+,27-,28+,30+,31+,32-;/m1./s1
• Clé InChI: SJCUTFKCLFLIFE-JWTJKVBLSA-N

Description

Description générale

CHAPS is a non-denaturing, zwitterionic sulfobetaine detergent. It is mild and solubilizes membrane proteins and receptors. It is useful for breaking protein-protein interactions. It is a derivative of the naturally occurring bile salts. It has small micellar molecular weight (6,150) and high critical micellar concentration (6 - 10 mM) allowing it to be removed from samples by dialysis.

Application

• 10mM CHAPS detergent has been used in the process of ion-exchange chromatography for improved purification.
• 0.5 w/v of CHAPS is a component of high salt buffer in protein purification alongside HEPES, NaCl and EDTA.
• HEPES buffer has been used as lysis buffer in immunoprecipitation, supplemented with 1% CHAPS.

Informations sur la sécurité

• Pictogrammes: GHS07
• Mention d'avertissement: Warning
• Mentions de danger: H302
• Conseils de prudence: P264 - P270 - P301 + P312 - P501
• Classification des risques: Acute Tox. 4 Oral
• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable