This item is stored refrigerated at 2-8°C. However, the product is shipped at ambient temperature. Shipping routes are configured to minimize transit time.
Refrigerated products that ship at ambient temperature are considered stable up to 2 weeks for transit time.
A7906
Albumine de sérum bovin
heat shock fraction, pH 7, ≥98%
Synonyme(s) :
Albumine bovine, Albumine, sérum bovin, BSA, ASB
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A propos de cet article
Numéro CAS:
UNSPSC Code:
12352202
EC Number:
232-936-2
NACRES:
NA.27
MDL number:
eCl@ss:
32160409
Biological source:
bovine
Assay:
≥98%
Form:
lyophilized powder
Technique(s):
blood typing: suitable, microbiological culture: suitable
Impurities:
≤5.0% Loss on drying
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Service technique
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Laissez-nous vous aiderbiological source
bovine
Quality Segment
assay
≥98%
form
lyophilized powder
purified by
heat shock fractionation
packaging
poly bottle of
origin
USA origin
technique(s)
blood typing: suitable, microbiological culture: suitable
impurities
≤5.0% Loss on drying
loss
≤5%
pH
7
solubility
water: soluble (40 mg/ml)
UniProt accession no.
foreign activity
BT virus, none detected, VSV virus, none detected
storage temp.
2-8°C
Gene Information
bovine ... ALB(280717)
Application
L'albumine de sérum bovin a été utilisée :
- comme constituant d′un mélange pour PCR (amplification en chaîne par polymérase)
- dans la préparation de neurones corticaux dissociés
- lors d′un marquage immunofluorescent
- comme constituant d′une solution saline exempte de Ca2+ (contenant de la collagénase, de la hyaluronidase et de la DNAse) destinée à la digestion de tissus bovins
Biochem/physiol Actions
Certains épitopes conformationnels et certains épitopes de la séquence primaire de l′ASB sont soupçonnés d′être des allergènes impliqués dans les allergies humaines au bœuf et au lait.
Preparation Note
L'albumine sérique est parfois appelée Fraction V. Cette dénomination provient de la méthode originelle de Cohn consistant à fractionner les protéines sériques par précipitation dans de l'alcool froid. L'albumine sérique a été trouvée dans la cinquième fraction d'éthanol selon la méthode de Cohn. Depuis, l'expression "Fraction V" est utilisée par certains pour décrire l'albumine sérique, quelle que soit la méthode de préparation employée. D'autres utilisent cette appellation pour décrire de l'albumine sérique purifiée par des méthodes de fractionnement à l'éthanol qui ont beaucoup évolué depuis le développement de la méthode de Cohn d'origine. Sigma-Aldrich fabrique et distribue des albumines sériques purifiées à l'aide de diverses méthodes primaires, notamment la véritable méthode de fractionnement de Cohn, des méthodes de fractionnement à l'éthanol modifiées, le choc thermique et la chromatographie. Les étapes de purification supplémentaires peuvent inclure une cristallisation ou une filtration sur charbon.
Produit préparé par fractionnement par choc thermique.
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Cet article | |||
|---|---|---|---|
| origin USA origin | origin USA origin | origin USA origin | origin USA origin |
| technique(s) blood typing: suitable, microbiological culture: suitable | technique(s) blood typing: suitable, microbiological culture: suitable | technique(s) ELISA: suitable, blocking: suitable, electrophoresis: suitable, western blot: suitable | technique(s) blood typing: suitable |
| assay ≥98% | assay ≥96% | assay ≥96% | assay ≥96% |
| biological source bovine | biological source bovine | biological source bovine | biological source bovine |
| Quality Level 300 | Quality Level 200 | Quality Level 300 | Quality Level 200 |
| form lyophilized powder | form lyophilized powder | form lyophilized powder | form lyophilized powder |
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Classe de stockage
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Numéro d'article de commerce international
| Référence | GTIN |
|---|---|
| A7906-5KG | 04061835515295 |
| A7906-10G | 04061835504510 |
| A7906-100G | 04061835521043 |
| A7906-500G | 04061835553631 |
| A7906-1KG | 04061835553624 |
| A7906-50G | 04061835511747 |
| A7906-250G | 04061833693377 |
| A7906-10KG | 04061835511730 |
| A7906-5G | 04061833693384 |
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How long is BSA (A7906) viable at room temperature? 200g was left on benchtop overnight (Approximately 22 hours), will it still work?
1 answer-
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Is the product A7906, Albumin from bovine serum (BSA), fatty acid-free?
1 answer-
This product is not considered to be fatty acid-free. Please see the link below to review available fatty acid-free product options:
https://www.sigmaaldrich.com/search/bovine-serum-albumin-fatty-acid-free?facet=facet_biological_source%3Abovine&focus=products&page=1&perpage=30&sort=relevance&term=bovine%20serum%20albumin%20fatty%20acid%20free&type=productHelpful?
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After reconstitution in PBS, for how long is the protein stable in solution if stored at 4°C or -20*C?
1 answer-
Prepared BSA solution products also available for purchase. These products have a shelf life ranging from 18 months to 2 years. Many are assigned a restest schedule with no firm expiration date. These products are prepared in saline, DPBS, or Tris. These solutions are filtered products and offered in a range of concentrations. The storage recommendation for these products is 2 - 8 deg. C. In addition, BSA is often used as a carrier protein for less stable compounds that are stored frozen. While there is no established shelf life for a BSA solution stored frozen, it is quite stable. See the link below for a sample Certificate of Analysis for one of the BSA solutions.
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Does this BSA have bovine immunoglobulins?
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This product does contain bovine immunoglobulins. BSA products that do not contain immunoglobulins will state "globulin-free" or "essentially globulin-free" on the product detail page. Product A3156 is an example of an immunoglobulin free BSA product.
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Is Product A7906, Albumin from bovine serum (BSA), suitable for use as a blocking agent in ELISA?
1 answer-
We have not tested the use of A7906 in ELISA. Because the remainder of the product is mostly globulins, we would not suggest A7906 for ELISA use.
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Can Product A7906, Albumin from bovine serum (BSA), be used in immunohistochemistry?
1 answer-
We have not ourselves tested A7906 for use in immunohistochemistry. However, a literature search can turn up publications that cite this use, such as this paper: Surendran, K., et al., Wnt-Dependent Beta-Catenin Signaling Is Activated after Unilateral Ureteral Obstruction, and Recombinant Secreted Frizzled-Related Protein 4 Alters the Progression of Renal Fibrosis. J. Am. Soc. Nephrol., Vol. 16, pp. 2373-2384 (2005).
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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Is Product A7906, Albumin from bovine serum (BSA), tested for insulin levels?
1 answer-
A7906 is not tested in quality control for insulin levels. One BSA that is indicated as "suitable for insulin RIA procedures" is A7888.
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Is Product A7906, Albumin from bovine serum (BSA), protease-free?
1 answer-
We have not tested A7906 in quality control to be protease-free. Other BSA products to suggest that are protease-free would be A3059 or B6917.
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