TAQL-RO

Taq DNA Polymerase, 1 U/μl

suitable for PCR, hotstart: no, dNTPs included: no

• Synonyme(s) : dna amplification, pcr, polymerase, primer extension
• Numéro de classification (Commission des enzymes): 2.7.7.7 (BRENDA, IUBMB)
• Code UNSPSC : 41106300

Propriétés

• Source biologique: bacterial (Thermus aquaticus BM)
• Niveau de qualité: 100
• Produit recombinant: expressed in E. coli
• Forme: liquid
• Utilisation: sufficient for ≤2,000 reactions (11647687001); sufficient for ≤500 reactions (11647679001)
• Activité spécifique: 1 U/μL
• Poids mol.: ~95 kDa
• Caractéristiques: dNTPs included: no; hotstart: no
• Conditionnement: pkg of 1,000 U (11647687001 [4 x 250 U]); pkg of 250 U (11647679001)
• Fabricant/nom de marque: Roche
• Concentration: 25 U/mL; 30 U/mL
• Paramètres: 72 °C optimum reaction temp.
• Technique(s): PCR: suitable
• Couleur: colorless
• Entrée: purified DNA
• pH optimal: ~9.0 (20 °C)
• Solubilité: water: miscible
• Adéquation: suitable for PCR; suitable for molecular biology
• Numéro d'accès NCBI: AAA27507; ; ; AAA27507
• Numéro d'accès UniProt: P19821
• Application(s): genomic analysis; life science and biopharma
• Activité étrangère: Endonucleases 20 units, none detected; Nicking activity 20 units, none detected
• Température de stockage: −20°C

Description

Description générale

The enzyme was cloned in E.coli and is isolated to be free of unspecific endo- or exonucleases according to the current quality control procedurese. Taq DNA Polymerase is a highly processive 5′–3′ DNA polymerase that lacks 3′–5′ exonuclease activity. It consists of a single polypeptide chain with a molecular weight of approximately 95 kDa. The enzyme exhibits highest activity at a pH of around 9 (adjusted at 20 °C) and temperatures around +75 °C. Taq DNA Polymerase also accepts modified deoxyribonucleosidetriphosphates as substrates, and can be used to label DNA-fragments either with radionucleotides, digoxigenin, fluorescein or biotin.
The high processivity, absence of exonuclease activity and temperature optima of Taq DNA Polymerase enable the use of this enzyme in DNA sequencing, especially where the resolution of secondary structures plays a major role.

Application

This lower concentration of our recombinant Taq DNA Polymerase allows small amounts of the polymerase to be pipetted more accurately and conveniently. In all other respects, this preparation is identical to our higher concentration (5 U/μl) preparation and can be used for:

• PCR
• RT-PCR
• Other primer-extension reactions, such as sequencing and labeling

Conditionnement

1 kit containing 4 components

Définition de l'unité

One unit Taq DNA Polymerase is defined as the amount of enzyme that incorporates 10 nmol of total deoxyribonucleoside triphosphates into acid precipitable DNA within 60 min at +65 °C under the assay conditions stated above.

Unit Assay: Incubation buffer:
67 mM Tris/HCl; pH 8.3/25 °C, 5 mM MgCl₂, 10 mM Mercaptoethanol, 0.2% Polydocanol, 0.2 mg/ml Gelatine, 0.2 mM each dATP, dGTP, dTTP and 0.1 mM dCTP.

Incubation procedure:
M13mp9ss, M13 primer (17mer) and 1 μCi (α^-32P) dCTP are incubated with suitable dilutions of Taq DNA Polymerase in 50 μl incubation buffer at +65 °C for 60 minutes. The amount of incorporated dNTPs is determined by trichloroacetic acid precipitation.

Volume Activity: 1 U/μl

Autres remarques

For life science research only. Not for use in diagnostic procedures.

Informations légales

Use of this product is covered by US patent claims and corresponding patent claims outside the US. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser′s own internal research. No right under any other patent claim, no right to perform any patented method, and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patent claims require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.

Composants de kit seuls

• Taq DNA Polymerase 1 U/μl
• PCR Buffer with MgCl₂ 10x concentrated
• MgCl₂ Stock Solution
• PCR Buffer without MgCl₂

Informations sur la sécurité

• Mentions de danger: H412
• Conseils de prudence: P273 - P501
• Classification des risques: Aquatic Chronic 3
• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 2
• Point d'éclair (°F): does not flash
• Point d'éclair (°C): does not flash