12140314001
Roche
High Fidelity PCR Master
sufficient for ≤200 reactions, kit of 1 (2 components), suitable for PCR
Synonyme(s) :
High fidelity PCR | PCR
Se connecterpour consulter vos tarifs contractuels et ceux de votre entreprise/organisme
About This Item
Code UNSPSC :
41106300
Produits recommandés
Utilisation
sufficient for ≤200 reactions
Niveau de qualité
Caractéristiques
High Fidelity PCR
dNTPs included
hotstart: no
Conditionnement
kit of 1 (2 components)
Fabricant/nom de marque
Roche
Technique(s)
PCR: suitable
Entrée
purified DNA
Température de stockage
2-8°C
Catégories apparentées
Description générale
The 5′→3′ polymerase activity of Taq DNA Polymerase and the 3′→ 5′ exonuclease of the proofreading polymerase combine for improved performance and results; 3′→5′ exonuclease proofreading produces a threefold increase in DNA synthesis fidelity compared to Taq DNA polymerase.
PCR products have both blunt ends and 3′ single A-overhangs just like using Taq DNA polymerase, ideal for blunt end cloning or T/A cloning.
The High Fidelity PCR Master is a ready-to-use, double-concentrated master mix that contains all the reagents required to perform PCR (except template and primer). Water, PCR Grade, is also supplied.
This powerful specific enzyme blend of the High Fidelity PCR Master is designed to give PCR products with high yield, high fidelity, and high specificity from episomal and genomic DNA.
The special enzyme blend is composed of two thermostable polymerases: Taq DNA Polymerase and a thermostable polymerase with proofreading activity. Due to the inherent 3′-5′ exonuclease activity of the proofreading polymerase, the High Fidelity PCR Master results in approximately three times the fidelity and approximately twice the yield of Taq DNA Polymerase alone.
PCR products have both blunt ends and 3′ single A-overhangs just like using Taq DNA polymerase, ideal for blunt end cloning or T/A cloning.
The High Fidelity PCR Master is a ready-to-use, double-concentrated master mix that contains all the reagents required to perform PCR (except template and primer). Water, PCR Grade, is also supplied.
This powerful specific enzyme blend of the High Fidelity PCR Master is designed to give PCR products with high yield, high fidelity, and high specificity from episomal and genomic DNA.
The special enzyme blend is composed of two thermostable polymerases: Taq DNA Polymerase and a thermostable polymerase with proofreading activity. Due to the inherent 3′-5′ exonuclease activity of the proofreading polymerase, the High Fidelity PCR Master results in approximately three times the fidelity and approximately twice the yield of Taq DNA Polymerase alone.
Application
- Amplify longer fragments with more fidelity
- Reduce setup time.
- Minimize contamination with two pipetting steps:
Choose the convenient High Fidelity PCR Master, a double-concentrated, ready-to-use mix, which combines Expand High Fidelity PCR System with PCR-Grade dNTPs, MgCl2, and an optimized reaction buffer. The High Fidelity PCR Master delivers twice the yield and three times better fidelity than Taq DNA Polymerase alone.High yield and increased fidelity compared to Taq DNA Polymerase. Use the High Fidelity PCR Master for:
High Fidelity PCR Master is stable at +2 to +8°C, allowing PCR without time-consuming reagent thawing.
- PCR up to 5 kb
- RT-PCR up to 5 kb
High Fidelity PCR Master is stable at +2 to +8°C, allowing PCR without time-consuming reagent thawing.
Conditionnement
1 kit containing 2 components.
Qualité
Each lot is PCR function tested using human genomic DNA and primers specific for a 4.8 kb fragment of the tPA gene.
Autres remarques
For life science research only. Not for use in diagnostic procedures.
Informations légales
Use of this product is covered by US patent claims and corresponding patent claims outside the US. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser′s own internal research. No right under any other patent claim, no right to perform any patented method, and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patent claims require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.</ br>NOTICE TO PURCHASER: LIMITED LICENSE<br />Use of this product is covered by US Patent No. 6,127,155 and corresponding patent claims outside the US. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser′s own internal research. No right under any other patent claim and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Human and veterinary diagnostic uses under Roche patent claims require a separate license from Roche. All uses other than internal research and human and veterinary diagnostic uses under Roche patent claims require a separate license from Thermo Fisher Scientific. Further information on purchasing licenses from Roche may be obtained by contacting the Licensing Department of Roche Molecular Systems, Inc., 4300 Hacienda Drive, Pleasanton, California 94588, USA or Roche Diagnostics GmbH, Sandhofer Strasse 116, 68305 Mannheim, Germany. Further information on purchasing licenses from Thermo Fisher Scientific may be obtained by contacting the Licensing Department of Thermo Fisher Scientific, 5791 Van Allen Way, Carlsbad, California 92008, USA.
Composants de kit seuls
Réf. du produit
Description
- High Fidelity PCR Master mix containing enzyme blend, reaction buffer, and nucleotides (10 vials)
- Water, PCR Grade (5 vials)
Code de la classe de stockage
12 - Non Combustible Liquids
Classe de danger pour l'eau (WGK)
WGK 1
Point d'éclair (°F)
does not flash
Point d'éclair (°C)
does not flash
Certificats d'analyse (COA)
Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".
Déjà en possession de ce produit ?
Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.
Les clients ont également consulté
Protocol for constructing large size human antibody heavy chain variable domain (VH) library and selection of SARS-CoV-2 neutralizing antibody domains
Chen, et al.
STAR protocols, 2, 17-17 (2021)
Santiago Plischuk et al.
Environmental microbiology reports, 1(2), 131-135 (2009-04-01)
As pollination is a critical process in both human-managed and natural terrestrial ecosystems, pollinators provide essential services to both nature and humans. Pollination is mainly due to the action of different insects, such as the bumblebee and the honeybee. These
Vincenzo Falbo et al.
Oncology reports, 26(1), 3-11 (2011-04-20)
Salivary gland tumours are rare tumours characterized by histopathologic complexity and a wide variety of morphologic features. Studies on genetic changes in different histological subtypes of salivary gland tumours are important to better understand molecular pathogenetic mechanisms and to identify
Cheng Li et al.
Cell, 185(8), 1389-1401 (2022-03-29)
The effectiveness of SARS-CoV-2 vaccines and therapeutic antibodies have been limited by the continuous emergence of viral variants and by the restricted diffusion of antibodies from circulation into the sites of respiratory virus infection. Here, we report the identification of
Articles
PCR master mix simplifies PCR/RT-PCR with components like DNA polymerase, dNTPs, MgCl2, and buffer, available commercially or DIY.
PCR master mix simplifies PCR/RT-PCR with components like DNA polymerase, dNTPs, MgCl2, and buffer, available commercially or DIY.
PCR master mix simplifies PCR/RT-PCR with components like DNA polymerase, dNTPs, MgCl2, and buffer, available commercially or DIY.
PCR master mix simplifies PCR/RT-PCR with components like DNA polymerase, dNTPs, MgCl2, and buffer, available commercially or DIY.
Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..
Contacter notre Service technique