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11175033910

Roche

DIG DNA Labeling Kit

greener alternative

sufficient for 40 labeling reactions, kit of 1 (7 components), suitable for hybridization

Synonyme(s) :

dig, dna labeling kit, dig

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About This Item

Code UNSPSC :
41105500

Utilisation

sufficient for 40 labeling reactions

Niveau de qualité

Conditionnement

kit of 1 (7 components)

Fabricant/nom de marque

Roche

Caractéristiques du produit alternatif plus écologique

Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.

sustainability

Greener Alternative Product

Technique(s)

hybridization: suitable

Autre catégorie plus écologique

Température de stockage

−20°C

Description générale

DIG DNA Labeling Kit is a convenient kit for the labeling of DNA with Digoxigenin-deoxyuridine triphosphate (dUTP) using random oligonucleotides as primers. In this method, the complementary DNA strand of denatured DNA is synthesized by Klenow polymerase using the 3′-OH termini of the random oligonucleotides as primers.
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product is designed as a safer chemical.  The DIG System was established as a sensitive and cost-effective alternative to radioactivity for the labeling and detection of nucleic acids. There are many available publications that prove the versatility of the DIG System, so use of radio-labeling is no longer the only option for labeling of DNA for hybridization.

Spécificité

Heat inactivation: Stop the reaction by adding 2 μl 0.2 M EDTA (pH 8.0) and/or by heating to 65 °C for 10 minutes.

Application

For Random-primed labeling of DNA probes with DIG-11-dUTP, alkali-labile. DIG-labeled DNA probes can be used for:
  • All types of filter hybridization according to our standard protocol given in the pack insert of the special hybridization solution DIG Easy Hyb.
  • Single-copy gene detection in total genomic DNA, even from organisms with high complexity, for example, human, barley, and wheat.
  • In situ hybridizations

Conditionnement

1 kit containing 7 components.

Qualité

Function tested in a Southern blot.

Caractéristiques

Assay Time: Labeling: 1hour to O/N
Sensitivity and specificity: A single-copy human gene (tPA gene) is detected with a DIG-labeled probe in a Southern blot of 1μg digested human placenta DNA.

Principe

DIG-labeled DNA probes are generated according to the random-primed DNA labeling method which is based on the hybridization of random oligonucleotides to the denatured DNA template. The complementary DNA strand is synthesized by Klenow enzyme which uses the 3′-OH termini of the random oligonucleotides as primers and a mixture of deoxyribonucleotides containing DIG-11-dUTP, alkali-labile for elongation. This results in incorporation of digoxigenin into the newly synthesized DNA.

Note:
  • The use of the alkali-labile form of DIG-11-dUTP enables easier and more efficient stripping of blots for rehybridization experiments with a second DIG-labeled probe.
  • DNA probe, labeled with DIG-11-dUTP, alkali-labile must not be denatured using NaOH, but can be denatured by boiling in a waterbath.

Autres remarques

For life science research only. Not for use in diagnostic procedures.

Composants de kit seuls

Réf. du produit
Description

  • Unlabeled Control DNA 1 100 µg/ml

  • Unlabeled Control DNA 2 100 µg/ml

  • DNA Dilution Buffer

  • DIG-labeled Control DNA 5.2 µg/ml

  • Hexanucleotide Mix 10x concentrated

  • dNTP Labeling Mixture 10x concentrated

  • Klenow Enzyme, Labeling grade 2 U/µl

Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

does not flash

Point d'éclair (°C)

does not flash


Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Yanfang Du et al.
The New phytologist, 214(2), 721-733 (2017-01-04)
UNBRANCHED3 (UB3), a member of the SQUAMOSA promoter binding protein-like (SPL) gene family, regulates kernel row number by negatively modulating the size of the inflorescence meristem in maize. However, the regulatory pathway by which UB3 mediates branching remains unknown. We
Pedro Vallecillo-García et al.
Nature communications, 8(1), 1218-1218 (2017-11-01)
Fibro-adipogenic progenitors (FAPs) are an interstitial cell population in adult skeletal muscle that support muscle regeneration. During development, interstitial muscle connective tissue (MCT) cells support proper muscle patterning, however the underlying molecular mechanisms are not well understood and it remains
Lisa-Marie Marschall et al.
International journal of molecular sciences, 23(9) (2022-05-15)
Function of mitochondria largely depends on a characteristic ultrastructure with typical invaginations, namely the cristae of the inner mitochondrial membrane. The mitochondrial signature phospholipid cardiolipin (CL), the F1Fo-ATP-synthase, and the 'mitochondrial contact site and cristae organizing system' (MICOS) complex are
Feng Wang et al.
PloS one, 9(3), e91591-e91591 (2014-03-19)
The rice white tip nematode Aphelenchoides besseyi, a devastating nematode whose genome has not been sequenced, is distributed widely throughout almost all the rice-growing regions of the world. The aims of the present study were to define the transcriptome of
Cristina Corral-Ramos et al.
Autophagy, 11(1), 131-144 (2015-01-07)
In the fungal pathogen Fusarium oxysporum, vegetative hyphal fusion triggers nuclear mitotic division in the invading hypha followed by migration of a nucleus into the receptor hypha and degradation of the resident nucleus. Here we examined the role of autophagy

Articles

Digoxigenin (DIG) labeling methods and kits for DNA and RNA DIG probes, random primed DNA labeling, nick translation labeling, 5’ and 3’ oligonucleotide end-labeling.

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