10791156001
Roche
Endoproteinase Glu-C (V8 Protease)
from Staphylococcus aureus V8
Synonyme(s) :
V8 protease, protease v8
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About This Item
Produits recommandés
Forme
lyophilized (salt-free)
Activité spécifique
20 U/mg
Poids mol.
30 kDa
Conditionnement
pkg of 2 mg
Fabricant/nom de marque
Roche
pH optimal
8.0-8.5
Catégories apparentées
Description générale
Approximately 20 U/mg lyophilizate at +25°C with Z-Phe-Leu-Glu-4-nitranilide as the substrate (approximately 500 U/mg lyophilizate at +37°C with casein as the substrate).
At 25 °C with Z-Phe-Leu-Glu-4-nitranilide as the substrate (approximately 500 U/mg lyophilizate at 37 °C with casein as the substrate).
Endoproteinase Glu-C is a Staphylococcal serine proteinase. Its inhibitors are DFP, α2-macroglobulin and TLCK.
Spécificité
Heat inactivation: Endoproteinase Glu-C is inactivated by boiling for ten minutes.
Application
Use Endoproteinase Glu-C (V8 Protease) for protein structure analysis and for sequence analysis.
Actions biochimiques/physiologiques
Endoproteinase Glu-C specifically hydrolyzes peptide and ester bonds at the carboxylic side of Glu, or both Glu and Asp, depending on the buffer used.
Notes préparatoires
Activator: The enzyme has its maximal activity in presence of SH-reagents
Working concentration: 1 to 5 mM
Working solution: Recommended solvent is 50 mM ammonium acetate pH 4.0 (2 mg/ml).
Storage conditions (working solution): -15 to -25 °C
The enzyme (2 mg/ml in 50 mM ammonium acetate, pH 4.0) is stable for at least one month, frozen in aliquots and thawed only once.
Working concentration: 1 to 5 mM
Working solution: Recommended solvent is 50 mM ammonium acetate pH 4.0 (2 mg/ml).
Storage conditions (working solution): -15 to -25 °C
The enzyme (2 mg/ml in 50 mM ammonium acetate, pH 4.0) is stable for at least one month, frozen in aliquots and thawed only once.
Stockage et stabilité
Store at 2 to 8 °C. (Store dry!)
Autres remarques
For life science research only. Not for use in diagnostic procedures.
Mention d'avertissement
Danger
Mentions de danger
Conseils de prudence
Classification des risques
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
Organes cibles
Respiratory system
Code de la classe de stockage
11 - Combustible Solids
Classe de danger pour l'eau (WGK)
WGK 1
Point d'éclair (°F)
does not flash
Point d'éclair (°C)
does not flash
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