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10165948001

Roche

RNA, MS2

from bacteriophage MS2

Synonyme(s) :

RNA, ribonucleic acid

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About This Item

Code UNSPSC :
41106311

Source biologique

bacteriophage MS2

Niveau de qualité

100

Forme

solution

Poids mol.

~1200 kDa

Conditionnement

pkg of 500 μL (10 A260 units)

Fabricant/nom de marque

Roche

Technique(s)

RNA extraction: suitable

Couleur

colorless

Solubilité

water: miscible

Température de stockage

−20°C (−15°C to −25°C)

Catégories apparentées

Description générale

RNA, from bacteriophage MS2 is composed of 3569 nucleotides. MS2 bacteriophage acts as a model system in studying molecular biology processes, such as viral RNA replication, process of translation and physiology of infected cells. MS2 RNA codes for three viral polypeptides namely, protein A, coat protein and RNA replicase complex. Protein A and coat protein make up the structure of MS2 virus.

Application

RNA, MS2 has been used:
  • as a standard to assess RNA quantification methods
  • in RNA extraction
  • in single-tube, fluorescent PERT assay (STF-PERT) for accurate quantitation of different retrovirus types
  • for studies which use natural RNA in a in vivo and in vitro protein-synthesizing system, initiation, elongation, and termination of polypeptide synthesis.
  • for structural and functional studies.
  • to stabilize RNA during cDNA synthesis
  • to improve RNA yield during RNA extraction or isolation

Qualité

Typical analysis: Free of protein and host nucleic acids according to current Quality Control procedures.

Séquence

Chain Length 3,569 nucleotides

Notes préparatoires

Working solution: Storage buffer: 10 mM Tris-HCl, 1 mM EDTA, pH 7.0.

Autres remarques

For life science research only. Not for use in diagnostic procedures.

Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

nwg

Point d'éclair (°F)

No data available

Point d'éclair (°C)

No data available

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Aitana Braza-Boïls et al.
International journal of molecular sciences, 21(8) (2020-04-25)
There is evidence for the effects of platelet inhibition on innate immune activation. Circulating microRNAs (miRNAs) have been implicated as markers of platelet and leukocyte activation. In the present study, we assessed the effects of P2Y12 inhibitors on platelet and
Michael Eikmans et al.
International journal of molecular sciences, 21(11) (2020-06-12)
About 10-15% of couples who want to conceive suffer from subfertility, while in 30% of these cases, a male factor plays a role. Levels of particular microRNAs in seminal plasma, including those involved in spermatogenesis, may serve as an indicative
Petra Vychytilova-Faltejskova et al.
Carcinogenesis, 37(10), 941-950 (2016-08-04)
Early detection of colorectal cancer is the main prerequisite for successful treatment and reduction of mortality. Circulating microRNAs were previously identified as promising diagnostic, prognostic and predictive biomarkers. The purpose of this study was to identify serum microRNAs enabling early
Shalini Das Gupta et al.
Scientific reports, 10(1), 9012-9012 (2020-06-04)
Quantification of plasma microRNAs (miRNAs) as non-invasive disease biomarkers is subject to multiple technical variabilities. This study aimed to develop an optimized protocol for miRNA quantification from rodent plasma. We hypothesized that a fixed small RNA concentration input for reverse
Roman Aranda et al.
Analytical biochemistry, 387(1), 122-127 (2009-05-21)
Quantification of RNA is essential for various molecular biology studies. In this work, three quantification methods were evaluated: ultraviolet (UV) absorbance, microcapillary electrophoresis (MCE), and fluorescence-based quantification. Viral, bacterial, and eukaryotic RNA were measured in the 500 to 0.05-ng microl(-1)
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