MABN1727
Anti-Beta-IV spectrin Antibody, clone N393/76
clone N393/76, from mouse
Synonyme(s) :
Spectrin beta chain, non-erythrocytic 4, Spectrin, non-erythroid beta chain 3
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About This Item
Code UNSPSC :
12352203
eCl@ss :
32160702
Nomenclature NACRES :
NA.43
Produits recommandés
Source biologique
mouse
Niveau de qualité
Forme d'anticorps
purified immunoglobulin
Type de produit anticorps
primary antibodies
Clone
N393/76, monoclonal
Espèces réactives
human, rat
Réactivité de l'espèce (prédite par homologie)
mouse (based on 100% sequence homology)
Technique(s)
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable
Isotype
IgG2bκ
Numéro d'accès NCBI
Numéro d'accès UniProt
Conditions d'expédition
ambient
Modification post-traductionnelle de la cible
unmodified
Informations sur le gène
human ... SPTBN1(6711)
Description générale
Spectrin beta chain, non-erythrocytic 4 (UniProt: Q9H254; also known as Beta-IV spectrin, Spectrin, non-erythroid beta chain 3) is encoded by the SPTBN4 (also known as KIAA1642, SPTBN3) gene (Gene ID: 57731) in human. Beta-IV Spectrin belongs to the spectrin family of proteins and is abundantly expressed in brain and pancreatic islets. It belongs to the spectrin family of proteins and contains two CH (calponin-homology) domains, one PH domain, and 18 spectrin repeats. It has been isolated as an interactor of the receptor tyrosine phosphatase ICA512. Spectrin molecule is assembled as a tetramer of two alpha and two beta subunits. A tight association between the amino-terminal domain of an alpha subunit and the carboxyl-terminal domain of a beta subunit forms a heterodimer. In brain, beta-IV Spectrin co-localizes with ankyrin-G 480/270-kDa at initial segments and nodes of Ranvier of myelinated neurons. In brain its highest expression is observed in the cell bodies of large myelinated neurons. It is not detected in non-neuronal cells, including glial and endothelial cells.Beta-IV Spectrin along with ankyrin-G is required for the anchoring of voltage-gated Na+ channels and for cell adhesion molecule anchoring to the actin cytoskeleton. (Ref.: Berghs, S et al. (2000). J. Cell Biol. 151(5): 985-1002).
Spécificité
Clone N393/76 specifically detects human and rat beta-IV Spectrin by immunohistochemistry. it targets a sequence within 192 amino acids in the C-terminal half of human beta-IV Spectrin.
Immunogène
A recombinant fusion fragment corresponding to 192 amino acids from the C-terminal half of human beta-IV spectrin.
Application
Anti-Beta-IV spectrin Antibody, clone N393/76, Cat. No. MABN1727, is a highly specific mouse monoclonal antibody that targets beta-IV spectrin and has been tested in Immunohistochemistry (Paraffin) and Western Blotting.
Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected beta-IV spectrin in human pancreas, human cervical cancer, and human uterus tissue.
Western Blotting Analysis: A representative lot detected beta-IV spectrin in COS cells transiently transfected with Myc-tagged beta 4-spectrin.
Immunohistochemistry Analysis: A representative lot detected beta-IV spectrin in rat cerebral cortex and hippocampus tissues.
Western Blotting Analysis: A representative lot detected beta-IV spectrin in COS cells transiently transfected with Myc-tagged beta 4-spectrin.
Immunohistochemistry Analysis: A representative lot detected beta-IV spectrin in rat cerebral cortex and hippocampus tissues.
Research Category
Neuroscience
Neuroscience
Qualité
Evaluated by Immunohistochemistry in human cerebellum tissue.
Immunohistochemistry Analysis: A 1:50 dilution of this antibody detected beta-IV spectrin in human cerebellum tissue.
Immunohistochemistry Analysis: A 1:50 dilution of this antibody detected beta-IV spectrin in human cerebellum tissue.
Description de la cible
288.98 kDa calculated.
Forme physique
Format: Purified
Protein G purified
Purified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Stockage et stabilité
Stable for 1 year at 2-8°C from date of receipt.
Autres remarques
Concentration: Please refer to lot specific datasheet.
Clause de non-responsabilité
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Code de la classe de stockage
12 - Non Combustible Liquids
Classe de danger pour l'eau (WGK)
WGK 1
Certificats d'analyse (COA)
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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.
Drew M Nassal et al.
The Journal of biological chemistry, 297(1), 100893-100893 (2021-06-22)
Fibrosis is a pronounced feature of heart disease and the result of dysregulated activation of resident cardiac fibroblasts (CFs). Recent work identified stress-induced degradation of the cytoskeletal protein βIV-spectrin as an important step in CF activation and cardiac fibrosis. Furthermore
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