MABF294

Anti-LAG3 Antibody, clone 8C7.1

culture supernatant, clone 8C7.1, from mouse

• Synonyme(s) : Lymphocyte activation gene 3 protein, LAG-3, Protein FDC, CD223, LAG3
• Code UNSPSC : 12352203
• eCl@ss : 32160702
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: mouse
• Niveau de qualité: 100
• Forme d'anticorps: culture supernatant
• Type de produit anticorps: primary antibodies
• Clone: 8C7.1, monoclonal
• Espèces réactives: human
• Technique(s): flow cytometry: suitable; western blot: suitable
• Isotype: IgG2aκ
• Numéro d'accès NCBI: NP_002277
• Numéro d'accès UniProt: P18627
• Conditions d'expédition: dry ice
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... LAG3(3902)

Description

Description générale

Lymphocyte activation gene 3 protein (UniProt P18627; also known as CD223, LAG-3, Protein FDC) is encoded by the LAG3 (also known as FDC) gene (Gene ID 3902) in human. LAG3 is a single-pass type I membrane protein that binds MHC class II molecules on antigen presenting cells with high affinity. LAG3 expression is induced after T cell activation and functions as a negative regulator of T cell function. LAG3 blockage is shown to augment T cell proliferation and cytokine production, and LAG3-deficient mice have a defect in the downregulation of T cell responses induced by staphylococcal enterotoxin B or by virus infection. In addition, LAG3 is expressed on activated regulatory T (Treg) cells at higher levels than on activated effector T cells. LAG3 blockage inhibits the suppressive activity of Treg cells. LAG3 is also upregulated on tumour-infiltrating lymphocytes (TILs), and LAG3 blockage can enhance anti-tumour T cell responses.

Immunogène

Mouse Fc-tagged recombinant human LAG3 extracellular fragment.

Application

Flow Cytometry Analysis: A 1:100 dilution of this antibody from a representative lot detected LAG3 in 1X10E6 Jurkat cells subjected to CD3/CD28 costimulation.

This Anti-LAG3 Antibody, clone 8C7.1 is validated for use in Western Blotting, Flow Cytometry for the detection of LAG3.

Qualité

Evaluated by Western Blotting in NK-92c ell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected LAG3 in 10 µg of NK-92 cell lysate.

Description de la cible

~60 kDa observed

Forme physique

Mouse monoclonal IgG2aκ antibody tissue culture supernatant with 0.05% sodium azide.

Autres remarques

Concentration: Please refer to lot specific datasheet.

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): WGK 2