MABC1191

Anti-Sodium-Iodide Symporter Antibody, clone ARN1

clone ARN1, from mouse

• Synonyme(s) : Sodium/iodide cotransporter, Na(+)/I(-) cotransporter, Na(+)/I(-) symporter, Solute carrier family 5 member 5
• Code UNSPSC : 12352203
• eCl@ss : 32160702
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: mouse
• Forme d'anticorps: purified immunoglobulin
• Type de produit anticorps: primary antibodies
• Clone: ARN1, monoclonal
• Espèces réactives: human
• Conditionnement: antibody small pack of 25 μg
• Technique(s): immunohistochemistry: suitable (paraffin); western blot: suitable
• Isotype: IgG2aκ
• Numéro d'accès NCBI: NP_000444.1
• Numéro d'accès UniProt: Q92911
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... SLC5A5(6528)

Description

Description générale

Sodium/iodide cotransporter (UniProt: Q92911; also known as Na(+)/I(-) cotransporter, Sodium-iodide symporter, Na(+)/I(-) symporter, Solute carrier family 5 member 5) is encoded by the SLC5A5 (also known as NIS) gene (Gene ID: 6528) in human. Sodium/iodide cotransporter is a multi-pass membrane protein that mediates iodide uptake in thyroid gland. It is expressed primarily in thyroid tissue, but is present in lower levels in mammary gland and ovary. It is found in the basolateral membrane of thyroid follicular cells where it mediates efficient iodide uptake from the bloodstream into the thyroid using the sodium gradient generated by Na+/K+-ATPase. It transports one iodide ion along with two sodium ions following the inwardly directed sodium gradient. Sodium/iodide cotransporter expression is significantly reduced in tumors. The presence of symporter in mammary glands leads to secretion of iodine in milk that is required for thyroid function in neonatal animals. NIS gene and protein expression is stimulated by thyroid-stimulating hormone. Both human and rat NIS contain N-glycosylation sites, however, glycosylation is not required for its full activity. Mutations in SLC5A5 gene are known to cause thyroid dyshormonogenesis (TDH1) that is characterized by the inability of the thyroid to maintain a concentration difference of readily exchangeable iodine between the plasma and the thyroid gland, leading to congenital hypothyroidism. Autoantibodies to NIS adversely affect iodide transport.

Spécificité

Clone ARN1 is a mouse monoclonal antibody that detects Sodium/iodide cotransporter in human cells. It targets an epitope within 13 amino acids from the C-terminal region.

Immunogène

Epitope: C-terminus

KLH-conjugated linear peptide corresponding to 13 amino acids from the C-terminal region of human Sodium/iodide cotransporter.

Application

Anti-Sodium-Iodide Symporter, clone ARN1, Cat. No. MABC1191, is a mouse monoclonal antibody that detects Sodium/iodide cotransporter and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting.

Immunohistochemistry (Paraffin) Analysis: A 1:250 dilution from a representative lot detected Sodium-Iodide Symporter in human stomach tissue sections.

Immunohistochemistry (Paraffin) Analysis: A representative lot detected Sodium-Iodide Symporter in Immunohistochemistry applications (Tazebay, U.H., et. al. (2000). 6(8):871-8).

Research Category
Apoptosis & Cancer

Qualité

Evaluated by Western Blotting in MDCK cells transduced with human WT NIS.

Western Blotting Analysis: 1 µg/mL of this antibody detected Sodium-Iodide Symporter in MDCK cells transduced with human WT Sodium-Iodide Symporter (NIS).

Description de la cible

~68 kDa observed; 68.67 kda calculated. Uncharacterized bands may be observed in some lysate(s).

Forme physique

Format: Purified

Protein G purified

Purified mouse monoclonal antibody IgG2a in buffer containing 140 mM Tris and 180 mM Glycine (pH 7.4) without azide.

Stockage et stabilité

Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Autres remarques

Concentration: Please refer to lot specific datasheet.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable