ECM540
ECM Cell Adhesion Array Kit, colorimetric
The ECM Cell Adhesion Array kit is useful for assessing specific cell surface integrins, monitoring in vitro cell differentiation, or screening potential cell adhesion promoters/inhibitors.
Synonyme(s) :
Extracellular matrix array cell adhesion assay
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About This Item
Code UNSPSC :
12352207
eCl@ss :
32161000
Nomenclature NACRES :
NA.32
Produits recommandés
Niveau de qualité
Espèces réactives
human
Fabricant/nom de marque
Chemicon®
Technique(s)
cell based assay: suitable
Méthode de détection
colorimetric
Conditions d'expédition
wet ice
Description générale
Introduction:
Cell adhesion plays a major role in cellular communication and regulation, and is of fundamental importance in the development and maintenance of tissues. Scientists are continually examining the adhesion, migration, and invasion of many diverse cell types on various extracellular matrix (ECM) component proteins. The CHEMICON ECM Cell Adhesion Array Kit (Colorimetric) provides an ECM Array microtiter plate with a colorimetric detection format, allowing for large-scale screening and quantitative comparison of multiple samples/cell lines. The kit is designed as a rapid (1-2 hours), cost effective, efficient method for characterization of cell adhesion.
In addition, Chemicon continues to provide numerous migration, invasion, and adhesion products including:
· Alpha Integrin-Mediated Cell Adhesion Array Kit (Colorimetric) (ECM530)
· Beta Integrin-Mediated Cell Adhesion Array Kit (Colorimetric) (ECM531)
· Alpha/Beta Integrin-Mediated Cell Adhesion Array Combo Kit (Colorimetric) (ECM532)
· Alpha Integrin-Mediated Cell Adhesion Array Kit (Fluorimetric) (ECM533)
· Beta Integrin-Mediated Cell Adhesion Array Kit (Fluorimetric) (ECM534)
· Alpha/Beta Integrin-Mediated Cell Adhesion Array Combo Kit (Fluorimetric) (ECM535)
· ECM Cell Adhesion Array Kit (Fluorimetric) (ECM545)
· QCM 8μm 96-well Chemotaxis Cell Migration Assay (ECM510)
· QCM 5μm 96-well Chemotaxis Cell Migration Assay (ECM512)
· QCM 3μm 96-well Chemotaxis Cell Migration Assay (ECM515)
· QCM 96-well Cell Invasion Assay (ECM555)
· QCM 96-well Collagen-Based Cell Invasion Assay (ECM 556)
· 24-well Insert Cell Migration and Invasion assay systems
· CytoMatrix Cell Adhesion strips (ECM protein coated)
· QuantiMatrix ECM protein ELISA kits
Test Principle:
The CHEMICON ECM Cell Adhesion Array kit utilizes a colorimetric detection format, allowing large-scale screening and quantitative comparison of multiple samples. Each kit contains a 96-well, microtiter plate consisting of 12 X 8-well removable strips. Each well within a strip (7 wells total) is pre-coated with a different ECM protein (see table below) along with one BSA-coated well (negative control). Experimental cells are seeded onto the coated substrates, where adherent cells are captured. Subsequently, unbound cells are washed away, and the adherent cells are fixed and stained. After stain extraction, the relative cell attachment is determined using absorbance readings.
Additional information about the ECM components provided in the kit:
Cell adhesion plays a major role in cellular communication and regulation, and is of fundamental importance in the development and maintenance of tissues. Scientists are continually examining the adhesion, migration, and invasion of many diverse cell types on various extracellular matrix (ECM) component proteins. The CHEMICON ECM Cell Adhesion Array Kit (Colorimetric) provides an ECM Array microtiter plate with a colorimetric detection format, allowing for large-scale screening and quantitative comparison of multiple samples/cell lines. The kit is designed as a rapid (1-2 hours), cost effective, efficient method for characterization of cell adhesion.
In addition, Chemicon continues to provide numerous migration, invasion, and adhesion products including:
· Alpha Integrin-Mediated Cell Adhesion Array Kit (Colorimetric) (ECM530)
· Beta Integrin-Mediated Cell Adhesion Array Kit (Colorimetric) (ECM531)
· Alpha/Beta Integrin-Mediated Cell Adhesion Array Combo Kit (Colorimetric) (ECM532)
· Alpha Integrin-Mediated Cell Adhesion Array Kit (Fluorimetric) (ECM533)
· Beta Integrin-Mediated Cell Adhesion Array Kit (Fluorimetric) (ECM534)
· Alpha/Beta Integrin-Mediated Cell Adhesion Array Combo Kit (Fluorimetric) (ECM535)
· ECM Cell Adhesion Array Kit (Fluorimetric) (ECM545)
· QCM 8μm 96-well Chemotaxis Cell Migration Assay (ECM510)
· QCM 5μm 96-well Chemotaxis Cell Migration Assay (ECM512)
· QCM 3μm 96-well Chemotaxis Cell Migration Assay (ECM515)
· QCM 96-well Cell Invasion Assay (ECM555)
· QCM 96-well Collagen-Based Cell Invasion Assay (ECM 556)
· 24-well Insert Cell Migration and Invasion assay systems
· CytoMatrix Cell Adhesion strips (ECM protein coated)
· QuantiMatrix ECM protein ELISA kits
Test Principle:
The CHEMICON ECM Cell Adhesion Array kit utilizes a colorimetric detection format, allowing large-scale screening and quantitative comparison of multiple samples. Each kit contains a 96-well, microtiter plate consisting of 12 X 8-well removable strips. Each well within a strip (7 wells total) is pre-coated with a different ECM protein (see table below) along with one BSA-coated well (negative control). Experimental cells are seeded onto the coated substrates, where adherent cells are captured. Subsequently, unbound cells are washed away, and the adherent cells are fixed and stained. After stain extraction, the relative cell attachment is determined using absorbance readings.
Additional information about the ECM components provided in the kit:
Application
Research Category
Cell Structure
Cell Structure
The CHEMICON ECM Cell Adhesion Array kit is useful for assessing specific cell surface integrins, monitoring in vitro cell differentiation, or screening potential cell adhesion promoters/inhibitors.
For Research Use Only. Not for use in diagnostic procedures.
For Research Use Only. Not for use in diagnostic procedures.
Conditionnement
96 wells
Composants
ECM Array Plate: (PN: 90652) One 96-well plate with 12 strips. Each 8-well strip consists of 7 different human, ECM protein-coated wells (Collagen I, Collagen II, Collagen IV, Fibronectin, Laminin, Tenascin, Vitronectin) and one BSA-coated well (negative control). See the plate layout on Data sheet.
Cell Stain Solution: (Part No. 90144) One bottle - 20 mL.
Extraction Buffer: (Part No. 90145) One bottle - 20 mL.
Assay Buffer: (Part No. 90601) One bottle - 100 mL.
Cell Stain Solution: (Part No. 90144) One bottle - 20 mL.
Extraction Buffer: (Part No. 90145) One bottle - 20 mL.
Assay Buffer: (Part No. 90601) One bottle - 100 mL.
Stockage et stabilité
The ECM Array Plate can be stored at 2° to 8°C in the foil pouch up to its expiration date. Unused strips may be placed back in the pouch for storage. Ensure that the desiccant remains in the pouch, and that the pouch is securely closed. Keep the remaining kit components at 2° to 8°C.
Precautions:
· Cell stain contains minor amounts of crystal violet, a toxic substance, which may cause cancer and heritable genetic damage. Handle with caution. Toxic by inhalation and if swallowed. Irritating to eyes, respiratory system and skin.
· Extraction buffer contains alcohol. Avoid internal consumption.
Precautions:
· Cell stain contains minor amounts of crystal violet, a toxic substance, which may cause cancer and heritable genetic damage. Handle with caution. Toxic by inhalation and if swallowed. Irritating to eyes, respiratory system and skin.
· Extraction buffer contains alcohol. Avoid internal consumption.
Informations légales
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Clause de non-responsabilité
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Mention d'avertissement
Danger
Mentions de danger
Conseils de prudence
Classification des risques
Eye Irrit. 2 - Flam. Liq. 2
Code de la classe de stockage
3 - Flammable liquids
Point d'éclair (°F)
53.6 °F
Point d'éclair (°C)
12 °C
Certificats d'analyse (COA)
Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".
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