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Key Documents

AB1837P

Sigma-Aldrich

Anti-Tumor Necrosis Factor-α Antibody

Chemicon®, from rabbit

Synonyme(s) :

TNF-alpha

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About This Item

Code UNSPSC :
12352203
eCl@ss :
32160702
Nomenclature NACRES :
NA.41

Source biologique

rabbit

Niveau de qualité

Forme d'anticorps

affinity purified immunoglobulin

Type de produit anticorps

primary antibodies

Clone

polyclonal

Produit purifié par

affinity chromatography

Espèces réactives

rat

Fabricant/nom de marque

Chemicon®

Technique(s)

ELISA: suitable
western blot: suitable

Numéro d'accès NCBI

Numéro d'accès UniProt

Conditions d'expédition

dry ice

Modification post-traductionnelle de la cible

unmodified

Informations sur le gène

human ... TNF(7124)

Spécificité

Reacts with rat TNF-alpha

Immunogène

Highly purified (>98%) recombinant rat TNF-alpha

Application

Anti-Tumor Necrosis Factor-α Antibody is an antibody against Tumor Necrosis Factor-α for use in ELISA & WB.
ELISA: 0.5 μg/mL this dilution will allow detection (in direct ELISA) of

0.2-0.4 ng/well when rat TNF-α is used to coat the plate.

Western blot: 0.1 - 0.2 μg/mL, this dilution should offer detection limit of 1.5 - 3.0 ng/lane, under reducing or non-reducing conditions.

Optimal working dilutions must be determined by the end user.
Research Category
Signaling
Research Sub Category
Growth Factors & Receptors

Liaison

Replaces: 04-1114

Forme physique

Affinity Purified Immunoglobulin. Lyophilized from PBS pH 7.2. Contains no preservative. Reconstitute with sterile distilled water.

Stockage et stabilité

The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile water to a concentration of =0.2 mg/ml the antibody is stable for at least six weeks at 2-4°C.

Autres remarques

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Informations légales

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Code de la classe de stockage

13 - Non Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable


Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

X J Fei et al.
Genetics and molecular research : GMR, 13(4), 10537-10544 (2014-12-17)
The dried roots of the plant Acanthopanax senticosus (AS) are used in traditional Oriental medicine and reportedly possess anti-inflammatory properties in vitro. However, whether AS has the same anti-inflammatory effect in vivo and the underlying mechanisms of this action remain
Fan Li et al.
Journal of neurochemistry, 106(5), 2093-2105 (2008-07-17)
Microglial cells are endowed with different potassium ion channels but their expression and specific functions have remained to be fully clarified. This study has shown Kv1.2 expression in the amoeboid microglia in the rat brain between 1 (P1) and 10
Clémence Guiraut et al.
International journal of molecular sciences, 17(12) (2016-11-30)
The medium-size intra-cranial arteries arising from the carotid bifurcation are prone to perinatal arterial ischemic strokes (PAIS). PAIS' physiopathology needs to be better understood to develop preventive and therapeutic interventions that are currently missing. We hypothesized that materno-fetal inflammation leads
Katherine Opalach et al.
Rejuvenation research, 13(1), 65-74 (2010-03-17)
Aging is associated with protein damage and imbalance in redox status in a variety of cells and tissues, yet little is known about the extent of age-related oxidative stress in the peripheral nervous system. Previously, we showed a drastic decline
Takehiko Yamanashi et al.
Scientific reports, 7(1), 7677-7677 (2017-08-11)
Neuro-inflammation has been shown to play a critical role in the development of depression. Beta-hydroxybutyrate (BHB) is a ketone body and has recently been reported to exert anti-inflammatory effects via inhibition of NLRP3 inflammasome. Here, we investigated the potential antidepressant

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