8.51209

D(+)-Biotin

Novabiochem^®

• Synonyme(s) : D(+)-Biotin, D-Biotin,Vitamin H, Hexahydro-2-oxothieno(3,4-d)-imidazole-4-pentanoic acid, Vitamin H
• Formule empirique (notation de Hill): C₁₀H₁₆N₂O₃S
• Numéro CAS: 58-85-5
• Poids moléculaire : 244.31
• Numéro MDL: MFCD00005541
• Code UNSPSC : 41116133
• Numéro de classement CE : 200-399-3
• Nomenclature NACRES : NA.22

Propriétés

• Niveau de qualité: 200
• Gamme de produits: Novabiochem^®
• Forme: solid
• Puissance: >2000 mg/kg LD₅₀, oral (Rat)
• Capacité de réaction: reaction type: Biotinylations
• Fabricant/nom de marque: Novabiochem^®
• pH: 4.5 (0.1 g/L in H₂O)
• Pf: 232-233 °C
• Solubilité: 0.2 g/L
• Masse volumique apparente: 200‑500 kg/m³
• Application(s): peptide synthesis
• Température de stockage: 2-8°C
• InChI: 1S/C10H16N2O3S/c13-8(14)4-2-1-3-7-9-6(5-16-7)11-10(15)12-9/h6-7,9H,1-5H2,(H,13,14)(H2,11,12,15)
• Clé InChI: YBJHBAHKTGYVGT-UHFFFAOYSA-N

Description

Description générale

D(+)-Biotin is the simplest reagent for the introduction of biotin to peptides, proteins and other biomolecules. In SPPS, it is best coupled using DIPCDI/Oxyma Pure. Biotin-labeled peptides have many important applications in immunology and histochemistry, such as affinity purification and FRET-based flow cytometry, solid-phase immunoassays, and receptor localization, that exploit the high affinity of streptavidin and avidin for biotin.^,,,

Associated Protocols and Technical Articles
Biotinylation Reagents for Peptide Synthesis

Literature references:
^[1] K. Hofmann & Y. Kiso (1976) Proc. Natl. Acad. Sci., 73, 3516.
^[2] T. Buranda, et al. (1999) Cytometry, 37, 21.
^[3] I. Sélo, et al. (1996) J. Immunol. Methods, 199, 127.
^[4] J. Howl, et al. (1993) Eur J Biochem., 213, 711.

Application

Some recent applications of D(+)-Biotin in SPPS:

• In the high-throughput biotinylation of proteins. The approach for site-specific biotinylation of proteins is useful for various applications such as protein purification and analysis.
• In the site-specific biotinylation of multipodal glycoclusters on a solid support. The synthesis of biotinylated glycoclusters, has important applications in glycobiology.

Informations légales

Novabiochem is a registered trademark of Merck KGaA, Darmstadt, Germany

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable