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71186

Millipore

YeastBuster Protein Extraction Reagent

Efficient extraction of protein from yeast without mechanical disruption and enzymatic lysis

Synonyme(s) :

Yeast protein extraction reagent

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About This Item

Code UNSPSC :
41106512
Nomenclature NACRES :
NA.77

Forme

liquid

Fabricant/nom de marque

Novagen®

Conditions de stockage

OK to freeze

Conditions d'expédition

wet ice

Catégories apparentées

Description générale







Efficient extraction of protein from yeast without mechanical disruption and enzymatic lysis
YeastBuster<TMSYMBOL></TMSYMBOL> Protein Extraction Reagent is formulated for a fast, efficient and gentle extraction of active proteins from yeast cells. The reagent avoids harsh conditions of vigorous mechanical or chemical treatment that often result in heat degradation of target proteins. The proprietary formulation utilizes a mix of mild detergent, protein stabilization buffer, and tris(hydroxypropyl) phosphine (THP) reducing agent (THP concentrate provided separately). This powerful combination eliminates the inconsistencies associated with tedious mechanical disruption of yeast cells with glass bead abrasives, ultrasonication and pressure disruption, or enzymatic digestion with β-1,3-glucanase lytic enzymes. The reagent has been tested with Saccharomyces cerevisiae, Pichia pastoris, P. stipidis, and Schizosaccharomyces pombe strains and with plant cells.

In practice, cells are harvested by centrifugation and suspended in YeastBuster. Following a brief incubation, insoluble cell debris is removed by centrifugation, and the clarified extract is ready to use. In addition to greater total protein yields in crude extracts and recovery of enzymatically active protein, the extracts are fully compatible with GST•Bind<TMSYMBOL></TMSYMBOL> and Ni-NTA HIS-BIND® immobilized metal affinity chromatography (IMAC) purification methods. The reagent is available in 100- and 500-ml sizes.

Caractéristiques et avantages

  • Gentle, rapid, efficient extraction of proteins from yeast cells
  • Eliminates the inconsistencies associated with abrasive grinding, ultrasonication, and pressure disruption of yeast cells
  • Higher yield of total and enzymatically active proteins
  • Fully compatible with Ni-NTA His•Bind and GST•Bind affinity purification methods

Composants

•100 ml or 500 mlYeastBuster Protein Extraction Reagent

•1 ml or 5 ml100X THP Solution

Avertissement

Toxicity: Multiple Toxicity Values, refer to MSDS (O)

Autres remarques

Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.

Informations légales

HIS-BIND is a registered trademark of Merck KGaA, Darmstadt, Germany
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

Pictogrammes

Health hazard
GHS08

Mention d'avertissement

Warning

Mentions de danger

H373

Conseils de prudence

P260 - P314 - P501

Classification des risques

STOT RE 2 Oral

Organes cibles

Kidney

Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

WGK 2

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

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Mark Zander et al.
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Articles

YeastBuster™ Protein Extraction Reagent for Fast, Efficient Extraction of Proteins from Yeast

YeastBuster™ Protein Extraction Reagent for Fast, Efficient Extraction of Proteins from Yeast

Contenu apparenté

Lyse et extraction des protéines

Panorama des méthodes de lyse cellulaire et d'extraction protéique, comme la solubilisation par détergents, la lyse par congélation/décongélation, le choc osmotique, la sonication, la lyse cellulaire enzymatique et les techniques de fragmentation mécanique comme l'homogénéisation au Dounce, au Polytron ou au mortier et au pilon.

Lyse et extraction des protéines

Panorama des méthodes de lyse cellulaire et d'extraction protéique, comme la solubilisation par détergents, la lyse par congélation/décongélation, le choc osmotique, la sonication, la lyse cellulaire enzymatique et les techniques de fragmentation mécanique comme l'homogénéisation au Dounce, au Polytron ou au mortier et au pilon.

Lysis & Protein Extraction

Cell lysis and protein extraction methods overview various techniques, from detergent solubilization to mechanical disruption, supporting research needs.

Lysis & Protein Extraction

Cell lysis and protein extraction methods overview various techniques, from detergent solubilization to mechanical disruption, supporting research needs.

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