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524627

Millipore

Phosphatase Inhibitor Cocktail III

liquid, for the inhibition of serine/threonine and protein tyrosine phosphatases

Synonyme(s) :

Phosphatase Inhibitor Mix (Set III), Phosphatase inhibitor

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About This Item

Code UNSPSC :
12352200
Nomenclature NACRES :
NA.77

product name

Phosphatase Inhibitor Cocktail Set III, Phosphatase Inhibitor Cocktail Set III is a ready to use cocktail of four phosphatase inhibitors for broad-spectrum inhibition of both serine/threonine and protein tyrosine phosphatases.

Forme

liquid

Fabricant/nom de marque

Calbiochem®

Conditions de stockage

OK to freeze

Conditions d'expédition

wet ice

Température de stockage

−20°C

Description générale

Phosphorylation and dephosphorylation regulate signal transduction processes involved in cellular activities such as cell division, proliferation, and apoptosis. Protein kinases add a phosphate group to specific amino acid residues (serine, threonine, or tyrosine) on target proteins, changing their conformation and interaction with ligands. Protein phosphatases remove the phosphate group, restoring the protein to its dephosphorylated state. When extracting phosphorylated proteins from cell and tissue lysates, it is crucial to maintain their phosphorylation status intact. Phosphatase Inhibitor Cocktail Set III is a cocktail of four phosphatase inhibitors for broad-spectrum inhibition of both serine/threonine and protein tyrosine phosphatases. Available as a 1 ml vial or as a set of five 1 ml vials.

Application

Phosphatase Inhibitor Cocktail Set III has been used:
  • as a supplement in lysis buffer to prepare protein lysates of whole testes for western blotting
  • to fortify ice-cold lysis radio-immunoprecipitation assay (RIPA) buffer to homogenize larval hindguts for western blot analysis
  • as a component in cell lysis buffer to extract total proteins for western blotting

Actions biochimiques/physiologiques

Cell permeable: no
Primary Target
Serine/threonine and protein tyrosine phosphatases
Product does not compete with ATP.
Reversible: no

Caractéristiques et avantages

  • Ready-to-use formulations for convenient use
  • High-quality products for consistent and reproducible performance,
  • Flexible options with a range of cocktails targeting different categories of phosphatases

Composants

Each vial contains 1 ml of aqueous solution with the following components:
  • 50 mM Sodium Fluoride
  • 10 mM ß-Glycerophosphate (Cat. No. 35675)
  • 10 mM Sodium Pyrophosphate Decahydrate
  • 1 mM Sodium Orthovanadate

Avertissement

Toxicity: Toxic (F)

Forme physique

In H₂O.

Reconstitution

For long-term storage, aliquot and freeze (-20°C). Dilute 1:5 just prior to use.

Informations légales

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

WGK 2

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable


Certificats d'analyse (COA)

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