516485

Pepstatin A Methyl Ester

A cell-permeable methyl ester derivative of Pepstatin A that acts as a potent, non-competitive, transition-state analog inhibitor of γ-secretase.

• Synonyme(s) : Pepstatin A Methyl Ester, Isovaleryl-V V-Sta-A-Sta-OCH₃, PME
• Formule empirique (notation de Hill): C₃₅H₆₅N₅O₉
• Poids moléculaire : 699.92
• Code UNSPSC : 12352108
• Nomenclature NACRES : NA.77

Propriétés

• Niveau de qualité: 100
• Pureté: ≥95% (HPLC)
• Forme: lyophilized
• Fabricant/nom de marque: Calbiochem^®
• Conditions de stockage: OK to freeze
• Solubilité: DMSO: 5 mg/mL
• Conditions d'expédition: wet ice
• Température de stockage: −20°C

Description

Description générale

A cell-permeable methyl ester derivative of Pepstatin A (Cat. No. 516481) that acts as a potent, non-competitive, transition-state analog inhibitor of γ-secretase (K_is = 150 nM, K_ii = 320 nM in solubilized human γ-secretase at 20°C; K_is is the inhibition constant for inhibitor binding to the free enzyme and K_ii is the inhibition constant for inhibitor binding to the Enzyme-Substrate complex). Sta = (3S,4S)-4-Amino-3-hydroxy-6-methyl-heptanoic acid.

A cell-permeable methyl ester derivative of Pepstatin A (Cat. No. 516482) that acts as a potent, non-competitive, transition-state analog inhibitor of γ-secretase (K_is = 15 nM and K_ii = 32 nM in solubilized human γ-secretase; K_is is the inhibition constant for inhibitor binding to the free enzyme and K_ii is the inhibition constant for inhibitor binding to the Enzyme-Substrate complex).

Actions biochimiques/physiologiques

Cell permeable: yes

Primary Target
γ-secretase

Product does not compete with ATP.

Reversible: no

Conditionnement

Packaged under inert gas

Avertissement

Toxicity: Standard Handling (A)

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C. Avoid freeze/thaw cycles of solutions.

Autres remarques

Tian, G., et al. 2002. J. Biol. Chem.277, 31499.
Zhang, L., et al. 2001. Biochemistry40, 5049.
Murphy, M.P., et al. 1999. J. Biol. Chem.274, 11914.

Informations légales

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable