492899

Nuclease, Staphylococcus aureus

Cleaves RNA and DNA to produce oligo- and mononucleotides with terminal 3′-phosphate groups.

• Synonyme(s) : Nuclease, Staphylococcus aureus
• Numéro CAS: 9013-53-0
• Numéro de classification (Commission des enzymes): 3.1.31.1 (BRENDA, IUBMB)
• Numéro MDL: MFCD00131731
• Code UNSPSC : 12352202
• Nomenclature NACRES : NA.54

Propriétés

• Source biologique: Staphylococcus aureus
• Niveau de qualité: 100
• Forme: lyophilized solid
• Activité spécifique: ≥15,000 units/mg protein
• Fabricant/nom de marque: Calbiochem^®
• Conditions de stockage: OK to freeze; desiccated (hygroscopic)
• Couleur: off-white
• Solubilité: aqueous buffer: 1 mg/mL
• Conditions d'expédition: ambient
• Température de stockage: 2-8°C

Description

Description générale

Native Stapylococcus aureus nuclease. Cleaves RNA and DNA to produce oligo- and mononucleotides with terminal 3′-phosphate groups. Also used to convert standard rabbit reticulocyte cell-free extracts (lysate) into mRNA-dependent protein synthesis systems. Note: 1 KU = 1000 units.

Avertissement

Toxicity: Standard Handling (A)

Définition de l'unité

One unit is defined as the amount of enzyme that will release a sufficient amount of acid-soluble oligonucleotides to produce an absorbance increase of 1 at 260 nm in 30 min at 37°C.

Autres remarques

Krupp, G., and Gross, H.J. 1979. Nucleic Acids Res.6, 3481.

Informations légales

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable